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Figure 4 (A) Methemoglobin assay of ACH and PRA effects on NO release from cultured bovine aortic endothelial cells. BAECs (2 ml) grown on microcarrier beads were perfused with oxyhemoglobin (3 µmol/liter) buffer containing 50 µmol/liter L-arginine at 2 ml/min and then stimulated with acetylcholine and pravastatin (1 and 10 µmol/liter, 3 min, n = 7). Open bars indicate basal NO release. Shaded bars indicate responses to agents above basal production. (*) Indicates difference from basal release (control); ( ) indicates difference from response at 0.1 µmol/liter p < 0.05. (B) Methemoglobin of BAEC responses to ACH and PRA in the presence of L-NAME and excess L-arginine as percent of control responses to these agents. Bars depict NO formation using the buffer above supplemented with L-NAME (1 µmol/liter, dark bar) or L-arginine (1 µmol/liter, light bar) stimulated with acetylcholine and pravastatin (1 and 10 µmol/liter, 3 min, n = 7). (*) indicates difference from control responses for that agent and dose; (#) indicates difference from responses with L-NAME and control response for that agent and dose. p < 0.05.
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