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Figure 3 (A) NO electrode assay of effects of ACH, PRA and SIM on NO release by cultured BAECs. Confluent cultures were prepared in 24-well plates and treated with 0.1 to 10 µmol/liter concentrations of ACH, PRA and SIM (open lactone ring preparation). Open bars indicate basal NO release. Shaded bars indicate responses to agents above basal production. (*) Indicates difference from basal release (control); ( ) indicates difference from response at 0.1 µmol/liter, p < 0.05. (B) NO electrode assay of L-NAME effects on BAEC responses to 1 µmol/liter of ACH, PRA and SIM (open ring preparation only) as a percent of control responses to these agents (A). (*) Indicates difference from control responses for that agent and dose. p < 0.05.
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