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Figure 1 Photomicrographs showing immunohistochemistry of ANP and BNP in human right atrial appendage (panels A through H). Note that panels A and C, panels B and D are serial sections from patient 14 in Table 1, panels E, F, G and H are serial sections from patient 1 in Table 1. Panels A and B show epicardial and endocardial side, respectively, in a section stained with anti-ANP antibody. Panels C and D, and panels E and F also show epicardial side and endocardial side, respectively, in each section stained with anti-BNP antibody. ANP-positive myocytes (brown staining) were seen from subendocardium (panel B) to subepicardium (panel A). The BNP-positive myocytes were seen in the subendocardial side of the atrial wall (panel D), and were not seen in subepicardial side (panel C) in patient 14. However, in patient 1, BNP-positive myocytes were seen in the subepicardial side as well as in the subendocardial side (panels E and F). Panel G (a serial section of panel E) shows ANP immunostaining. A control section (a serial section of panels E and G) incubated with anti-BNP antibody preabsorbed with BNP (panel H) showed no immunoreactivity. Asterisks and arrowheads indicates epicardial side and endocardial side, respectively. Original magnification was x200 (panels A through H).
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