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Figure 1 A, Region of interest (sampling field) of an endomyocardial biopsy specimen (Masson trichrome stain). B, Perpendicular measurement lines were placed across sectioned horizontal cardiomyocytes at the level of midnucleus. The line lengths were measured and averaged by computer software to provide the mean myocyte diameter of the biopsy specimen. C, Using the operator’s threshold settings, the myocardial fibrous tissue was highlighted (blue) and measured by computer software as percent of myocardial tissue. The white nontissue spaces were similarly demarcated, measured and subtracted to provide a corrected area of true myocardial tissue.
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