High Telomerase Activity in Neutrophils From Unstable Coronary Plaques
Maria Lucia Narducci, MD*,*,
Annalisa Grasselli, PhD*,¶,
Luigi Marzio Biasucci, MD, FACC*,
Antonella Farsetti, MD||,¶,
Antonino Mulè, MD ,
Giovanna Liuzzo, MD*,
Giuseppe La Torre, MD ,
Giampaolo Niccoli, MD*,
Rocco Mongiardo, MD*,
Alfredo Pontecorvi, MD and
Filippo Crea, MD, FACC*
* Institute of Cardiology
Division of Anatomic Pathology and Histology
Institute of Medical Pathology
Institute of Hygiene, Catholic University of Sacred Heart, Rome, Italy
|| Neurobiology and Molecular Medicine Institute, National Council of Research, Rome, Italy
¶ Department of Experimental Oncology, Regina Elena Cancer Institute, Rome, Italy
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Figure 1 Immunostaining of Coronary Plaque PMN With Myeloperoxidase Antibodies
Immunostaining for myeloperoxidase MPO-7 antibodies (purple) reveals staining of all cells isolated by direct washing of angioplasty balloon used for coronary revascularization, indicating that MPO-7–positive cells are neutrophils (arrow). Original magnification: x444. PMN = polymorphonuclear neutrophils.
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Figure 2 High Telomerase Activity in Coronary Plaque PMN of Patients With UA
Cell extracts derived from polymorphonuclear neutrophils (PMN) isolated from arterial (A) or venous blood (V) or directly from angioplasty balloons (AB) of unstable angina (UA) and chronic stable angina (SA) patients were assayed for telomerase activity by telomeric repeat amplification protocol assay in the presence of internal control (IC) (36 base pairs). As positive and negative controls, cell extracts (0.1 µg) from telomerase-positive HeLa cells were assayed before and after heat inactivation (HeLa and HeLa H.I.), respectively. Panels relative to the IC are shown at a lower detection exposure. Telomerase activity was barely detectable in PMN of patients with chronic SA (lanes 1 to 6, 13, and 14) and in arterial and venous PMN in patients with UA (lanes 8 and 9 and 11 and 12). In contrast, telomerase activity was high in PMN from the coronary atherosclerotic plaques in patients with UA (lanes 7 and 10).
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Figure 3 Distribution of Telomerase Activity
Telomerase activity in polymorphonuclear neutrophils derived from venous blood (V) or from washing medium of angioplasty balloons (AB) is represented as dot plots.
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Figure 4 Linear Regression Between Telomerase Activity And Timing of PCI
Among unstable angina patients, a significant inverse correlation was found between telomerase activity (ln: natural logarithm) in polimorphonuclear neutrophils obtained by atherosclerotic plaques and time interval (h) between last anginal episode and polimorphonuclear neutrophils sampling, during percutaneous coronary intervention (PCI).
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