Circulating Progenitor Cells Can Be Reliably Identified on the Basis of Aldehyde Dehydrogenase Activity
Thomas J. Povsic, MD, PhD*,*,
Katherine L. Zavodni, BS*,
Francine L. Kelly, BS*,
Shoukang Zhu, PhD*,
Pascal J. Goldschmidt-Clermont, MD, FACC ,
Chunming Dong, MD* and
Eric D. Peterson, MD, MPH, FACC
* Division of Cardiology, Duke University Medical Center, Durham, North Carolina
Duke Clinical Research Institute, Duke University Medical Center, Durham, North Carolina
Miller School of Medicine, University of Miami, Miami, Florida
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Figure 1 EPC Identification Based on ALDH Activity
A discrete group of cells, absent in the diethylaminobenzaldehyde (DEAB) control sample (left), with high aldehyde dehydrogenase (ALDH) activity and low side-scatter, is identified in the right lower quadrant (right). EPC = endogenous progenitor cell; FITC = fluorescein isothiocyanate.
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Figure 2 Characterization of ALDHbr Cells
Aldehyde dehydrogenase-bright (ALDHbr) cells were subjected to staining for expression of select endogenous progenitor cell markers (n = 21). The purple column indicates the percentage of CD133+CD34+ cells also expressing elevated ALDH activity.
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Figure 3 Correlation Between ALDHbr Cell Numbers and EPCs Expressing Specific Cell Surface Markers
Correlation of aldehyde dehydrogenase-bright (ALDHbr) cells with CD133+ cells (A), CD34+ cells (B), CD133+CD34+ cells (C), and vascular endothelial growth factor receptor (VEGFR)-2+CD34+ cells (D). Spearman correlation coefficients are shown. EPCs = endogenous progenitor cells; MNCs = mononuclear cells.
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Figure 4 Correlation of ALDHbr Cell Number and Patient Age and Extent of CAD
Aldehyde dehydrogenase-bright (ALDHbr) cells are inversely correlated with patient age (n = 110; p < 0.01) and extent of coronary artery disease (CAD) (n = 110; p value for analysis of variance analysis and analysis for linear trend). MNCs = mononuclear cells.
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