Important Role of Endogenous Hydrogen Peroxide in Pacing-Induced Metabolic Coronary Vasodilation in Dogs In Vivo
Toyotaka Yada, MD, PhD*,*,
Hiroaki Shimokawa, MD, PhD ,
Osamu Hiramatsu, PhD*,
Yoshiro Shinozaki, BS ,
Hidezo Mori, MD, PhD ,
Masami Goto, MD, PhD*,
Yasuo Ogasawara, PhD* and
Fumihiko Kajiya, MD, PhD*
* Department of Medical Engineering and Systems Cardiology, Kawasaki Medical School, Kurashiki, Japan
Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan
Department of Physiology, Tokai University School of Medicine, Isehara, Japan
Department of Cardiac Physiology, National Cardiovascular Center Research Institute, Suita, Japan
View larger version (22K):
[in this window]
[in a new window]
[Download PPT slide]
|
Figure 2 Coronary Vascular Responses to Cardiac Pacing
The coronary vasodilating responses of both-sized coronary arteries were significantly inhibited in all experimental conditions except L-NMMA alone. **p < 0.01. Abbreviations as in Figure 1.
|
|
View larger version (45K):
[in this window]
[in a new window]
[Download PPT slide]
|
Figure 3 Detection of H2O2 Production With DCF Fluorescent Method
Hydrogen peroxide (H2O2) production was unaltered after NG-monomethyl-L-arginine (L-NMMA) but was markedly suppressed by catalase. Number of arterioles/animals used was 5/5 for each group. *p < 0.05, **p < 0.01. DCF = 2',7'-dichlorodihydrofluorescein diacetate.
|
|
View larger version (47K):
[in this window]
[in a new window]
[Download PPT slide]
|
Figure 4 Detection of NO Production With DAR Fluorescent Method
Nitric oxide (NO) production was unaltered after catalase but was markedly suppressed by NG-monomethyl-L-arginine (L-NMMA). Number of arterioles/animals used was 5/5 for each group. *p < 0.05, **p < 0.01. DAR = diaminorhodamine-4M AM.
|
|
|
