Cellular Origins and Thrombogenic Activity of Microparticles Isolated From Human Atherosclerotic Plaques
Aurélie S. Leroyer, BSc*,
Hirotaka Isobe, PhD ,
Guy Lesèche, MD, PhD ,
Yves Castier, MD, PhD,
Michel Wassef, PhD ,
Ziad Mallat, MD, PhD*,
Bernd R. Binder, MD, PhD,
Alain Tedgui, PhD* and
Chantal M. Boulanger, PhD*,*
* INSERM Cardiovascular Research Center Lariboisière, Paris, France
Department of Vascular Biology and Thrombosis Research, Medical University, Vienna, Austria
Vascular Surgery Department, Beaujon Hospital, Clichy, France
Lariboisière Hospital, Paris, France
View larger version (22K):
[in this window]
[in a new window]
[Download PPT slide]
|
Figure 1 Plaque Microparticle Identification by Electron Microscopy and Cytofluorometry Analysis
Electron microscopy (A) and cytofluorometry analysis (B and C). (B) Plaque microparticles and calibrator beads (CAL) (10 µm diameter) are visualized in a forward scatter (FS)/side scatter (SS) logarithmic representation. Microparticles are defined as events (size 0.1 to 1 µm) gated in the "B" window. (C) Representative FL1 histogram of size-selected events versus fluorescence for AnnexinV-FITC binding (FL1). The yellow peak corresponds to negative control.
|
|
View larger version (12K):
[in this window]
[in a new window]
[Download PPT slide]
|
Figure 2 AnnexinV-Positive MPs
AnnexinV-positive macroparticle (MP) levels in plaque compared with the arterial wall adjacent to the lesion (n = 4) and to microparticles isolated from human mammary arteries (n = 3) using the same isolation procedure. *p < 0.0001 (Mann-Whitney U test).
|
|
View larger version (17K):
[in this window]
[in a new window]
[Download PPT slide]
|
Figure 3 Cellular Origin of Plaque MPs
Microparticle (MP) distribution and cellular origins in human plaque (A), venous plasma (B), and arterial plasma (C) (n = 26). Data are given as median (horizontal bar), 25th and 75th percentile (boxes), and 90th percentile (error bar). Endoth. = endothelial cell origin; Granul. = granulocyte origin; Lymph. = lymphocyte origin; Mac. = macrophage origin; Platel. = platelet origin; RBC = red blood cell origin; SMC = smooth muscle cell origin.
|
|
View larger version (15K):
[in this window]
[in a new window]
[Download PPT slide]
|
Figure 5 Plasma and Plaque MP Thrombin-Generating Activity
(A) Representative trace of thrombin generation by plasma and plaque microparticles (MPs) isolated from the same patient. (B) Peak thrombin generation by venous, arterial, and plaque microparticles (n = 18, 15, and 20, respectively). (C) Total thrombin generated (area under curve) for the same samples as in panel B. (D) Peak thrombin generated by 105 cell-derived microparticles. *p < 0.05 (analysis of variance followed by Bonferonni post-test; 3 comparisons in panels B and C; 10 in panel D). EC = endothelial cell–derived; PLT = platelet-derived; RBC = red blood cell–derived; SMC = smooth muscle cell–derived.
|
|
|
