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The Number of Endothelial Progenitor Cell Colonies in the Blood Is Increased in Patients With Angiographically Significant Coronary Artery Disease

Hasan Güven, MD^_*, Rebecca M. Shepherd, MD, Richard G. Bach, MD, FACC^_*, Benjamin J. Capoccia, BS and Daniel C. Link, MD^,_*

^_* Cardiology
Rheumatology
Oncology, Washington University School of Medicine, St. Louis, Missouri

View larger version (121K): [in a new window]   Figure 1 Phenotype of circulating angiogenic cells (CAC) and endothelial progenitor cells (EPC). Representative photomicrographs of cells on day 7 (CAC) or day 21 (EPC) of culture are shown in the left and right panels, respectively. The top panel shows phase contrast images; the middle panel demonstrates staining for Ulex-lectin; and the lower panel shows DiI-labeled acetylated-low density lipoprotein uptake (LDL-U). Original magnification x100.

View larger version (31K): [in a new window]   Figure 2 Cell surface phenotype of circulating angiogenic cells and endothelial progenitor cells. (A) Representative forward scatter (FSC-H) and side scatter (SSC-H) histograms of cultures on day 7 and 21 of culture. The gates used to analyze cells are shown. (B) Representative histograms of cells analyzed on day 7 (upper panel) and day 21 (lower panel). Isotype controls are shown as green lines.

View larger version (15K): [in a new window]   Figure 3 Number of endothelial progenitor cells (EPC) (A and C) and circulating angiogenic cells (CAC) (B and D) isolated from patients without and with significant coronary artery disease (stenosis <70% vs. 70%; A and B), and among patients stratified by maximum coronary stenosis <30%, 30% to 70%, and 70% (C and D), as assessed by coronary angiography.

View larger version (11K): [in a new window]   Figure 4 Correlation of endothelial progenitor cell (EPC) (A) and circulating angiogenic cell (CAC) (B) number with maximum angiographic percent diameter coronary stenosis. Spearman rank correlation was used to assess statistical significance.

View larger version (22K): [in a new window]   Figure 5 Number of endothelial progenitor cells (EPC) (A and C) and circulating angiogenic cells (CAC) (B and D) observed in patients stratified by number of vessels with stenosis 70% (A and B), and by subsequent clinical decision for revascularization (C and D). Revasc = revascularization; W = number of vessels with stenosis 70%.

View larger version (9K): [in a new window]   Figure 6 Correlation of endothelial progenitor cell (EPC) (A) and circulating angiogenic cell (CAC) (B) number with high-sensitivity C-reactive protein (hsCRP).