Aging Mouse Hearts Are Refractory to Infarct Size Reduction With Post-Conditioning
Karin Przyklenk, PhD*, , ,*,
Michelle Maynard*,
Chad E. Darling, MD* and
Peter Whittaker, PhD*,,,
* Department of Emergency Medicine, University of Massachusetts Medical School, Worcester, Massachusetts
Department of Anesthesiology, University of Massachusetts Medical School, Worcester, Massachusetts
Department of Medicine (Cardiology), University of Massachusetts Medical School, Worcester, Massachusetts
Cardiovascular Research Institute, Wayne State University School of Medicine, Detroit, Michigan.
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Figure 1 Protocol 1, Adult Mouse Hearts
(A) Infarct size (expressed as a percent of the total left ventricle [LV]). (B) Expression of phospho-Akt, phospho-MEK 1/2, and phospho-ERK 1/2. Data shown as mean ± SEM. ERK = extracellular-signal regulated kinase; MEK = mitogen-activated protein kinase kinase; PostC = post-conditioned; PD = PD98059. *p < 0.05 versus sham. p < 0.05 versus control. p = 0.07 versus control.
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Figure 2 Protocol 2, Old Mouse Hearts
(A) Infarct size (expressed as a percent of the total left ventricle [LV]). (B) Expression of phospho-Akt, phospho-MEK 1/2, and phospho-ERK 1/2. Data shown as mean ± SEM. *p < 0.05 versus sham. p < 0.05 versus control. Abbreviations as in Figure 1.
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Figure 3 Protocol 3, Role of MKPs
(A) Expression of MKP-1 in adult mouse hearts, 2-year-old mouse hearts, and 2-year-old mouse hearts infused with sodium orthovanadate (NaV). *p < 0.05 versus adult. (B) Expression of phospho-ERK 1/2 in 2-year-old mouse hearts infused with NaV. p = 0.08 versus NaV + control. (C) Infarct size (expressed as a percent of the total left ventricle [LV]) in 2-year-old mouse hearts infused with NaV. *p < 0.05 versus NaV + control. All data shown as mean ± SEM. Abbreviations as in Figure 1.
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