The pGL3 luciferase reporter contained either the T (pGL3-T) or C allele (pGL3-C) at the promoter –604 locus. Values represent the average of 6 experiments and the bars represent the standard deviation. The pGL3-basic was used as a negative control without any promoter sequence, and pGL3-control as a positive control. KDR = kinase insert domain-containing receptor; SNP = single nucleotide polymorphism.

HEK293s cells were transfected with 8 µg of pcDNA3.1-KDR (KDR-VH, KDR-IH, KDR-VQ, or KDR-IQ). After 36 h, the cells were rinsed with cold phosphate-buffered saline 3 times, and the binding of vascular endothelial growth factor (VEGF)₁₆₅ (10 ng/ml, R&D Systems, Minneapolis, Minnesota) was carried out in binding buffer containing DMEM, 25 mmol/l HEPES (pH 7.4), 1 µg/ml heparin, and 0.1% gelatin for 2 h at 4°C. Then the cells were rinsed with cold phosphate-buffered saline 5 times, lysed with cell lysis buffer, followed by enzyme-linked immunosorbent assay. The values were the ratio of VEGF to KDR. The experiments were repeated 3 times, and 2 replicates were performed for each experiment. The values were presented as means, and the T bars represented standard deviations. Abbreviations as in Figure 1.

Levels of the serum KDR were presented as means, and the T bars represented standard deviations. The correlation was significant (p = 0.013), and Spearman coefficient for the existing correlation was rs = –0.374. Nineteen samples with TT genotype, 14 with TC genotype, and 10 with CC genotype were analyzed. Abbreviations as in Figure 1.