Platelet P-selectin plays an important role in arterial thrombogenesis by forming large stable platelet-leukocyte aggregates
Shinji Yokoyama, MD*,
Hisao Ikeda, MD, PhD*,*,
Nobuya Haramaki, MD, PhD*,
Hideo Yasukawa, MD, PhD*,
Toyoaki Murohara, MD, PhD and
Tsutomu Imaizumi, MD, PhD, FACC*
* Department of Internal Medicine III and the Cardiovascular Research Institute, Kurume University School of Medicine, Kurume, Japan
Department of Cardiology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
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Figure 1 Representative tracings of carotid arterial flow. Note that time to thrombotic occlusion (TTO) was longer in a P-selectin-deficient (P–/–) mouse than in a wild-type (P+/+) mouse. Spontaneous reflow after total thrombotic occlusion was observed only in a P–/– mouse but not in a P+/+ mouse. FeCl3 = ferric chloride.
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Figure 2 Representative photographs of ferric chloride (FeCl3)-applied carotid arterial tissues stained with hematoxylin-eosin (A to D) and van Gieson’s elastin staining (F and G). In intact mice, endothelial cells were preserved in wild-type (P+/+) and P-selectin-deficient (P–/–) mice. However, in FeCl3-applied mice, almost no endothelial cells were observed in P+/+ and P–/– mice. Note that the number of endothelial cells in P+/+ and P–/– mice was significantly decreased by FeCl3 application and they were comparable (E). Furthermore, no disruptions of elastic fiber after FeCl3 application were observed in P+/+ and P–/– mice (F and G).
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Figure 3 Representative photographs of ferric chloride (FeCl3)-applied carotid arterial tissues stained with hematoxylin-eosin (A and B). Note that the number of leukocytes (arrows) within thrombi was significantly less in P-selectin-deficient (P–/–) mice than in wild-type (P+/+) mice (C).
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Figure 4 Platelet P-selectin expressions in vivo in wild-type (P+/+) and P-selectin-deficient (P–/–) mice (A and B). Ferric chloride (FeCl3) application increased in vivo expressions of platelet P-selectin only in P+/+ mice but not in P–/– mice (C). Platelet P-selectin expressions in P–/– mice were similar to those in the negative control (fluorescein isothiocyanate [FITC] immunoglobulin G [IgG]).
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Figure 5 (A and B) Adenosine diphosphate (ADP)-induced platelet and whole blood aggregations, respectively in wild-type (P+/+) and P-selectin-deficient (P–/–) mice. (C and D) Size distributions of whole blood aggregates in P+/+ and P–/– mice, respectively. (E and F) Representative two-color analyses of large platelet-leukocyte aggregate in P+/+ and P–/– mice, respectively.
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