HOME SUBSCRIPTIONS CURRENT ISSUE PAST ISSUES CARDIOSOURCE SEARCH HELP FEEDBACK		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Methe, H. Articles by Edelman, E. R. Search for Related Content PubMed PubMed Citation Articles by Methe, H. Articles by Edelman, E. R.

Enhanced T-Helper-1 Lymphocyte Activation Patterns in Acute Coronary Syndromes

Heiko Methe, MD^_*,,_*, Stefan Brunner^_*, Daniela Wiegand^_*, Michael Nabauer, MD^_*, Joerg Koglin, MD^_* and Elazer R. Edelman, MD, PhD

^_* Department of Cardiology, University Hospital Grosshadern, Ludwig-Maximilians-University, Munich, Germany
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, and the Cardiovascular Division, Department of Internal Medicine, Brigham and Women’s Hospital, Boston, Massachusetts

View larger version (14K): [in a new window]   Figure 1 Frequency of interferon (IFN)-+/CD3+ T cells in relationship to diameter of stenosis and to markers of myocardial damage. Results are shown as mean values ± SD or as median and range. Closed squares = IFN-/CD3+ cells; closed circles = diameter stensosis (DS); open diamonds = creatine kinase (CK); open trianges = troponin I. SA = stable angina pectoris; STEMI = ST-segment elevation myocardial infarction; UA = unstable angina pectoris; UH = unheralded myocardial infarction.

View larger version (40K): [in a new window]   Figure 2 Representative plots from a control patient (A) and a patient with stable angina pectoris (B), acute coronary syndrome (C), and unheralded myocardial infarction (D). Ten thousand CD3+ cells were gated and analyzed for expression of interferon (IFN)- or interleukin (IL)-4. Each dot represents a CD3+ T cell. Numbers represent the percentage of cells in the quadrants, respectively.

View larger version (17K): [in a new window]   Figure 3 Peripheral blood mononuclear cells from control patients and from patients with stable angina pectoris (SA), unstable angina pectoris (UA), ST-segment elevation myocardial infarction (STEMI), and unheralded myocardial infarction (UH) were immunostained with antibodies against CD3 and interferon (IFN)- and analyzed by flow cytometry. Symbols = individual data; dashed lines = mean values ± SD.

View larger version (52K): [in a new window]   Figure 4 (A) Representative examples of reverse transcription polymerase chain reaction amplification products of each group. 1, control; 2, stable angina; 3, unstable angina; 4, ST-segment elevation myocardial infarction; 5, unheralded myocardial infarction. (B) Reverse transcription polymerase chain reaction amplification was normalized against glyseraldehyde-3-phosphate dehydrogenase and is presented as mean corrected transcript levels ± SD in relative units (RU) of duplicate analysis of all patients in each group.*p < 0.002 vs. control, SA, and UH; p < 0.05 vs. control patients. GAPDH = glyceraldehyde-3-phosphate dehydrogenase; IFN = interferon; IL = interleukin; SA = stable angina pectoris; STAT = signal transducer and activator of transcription; STEMI = ST-segment elevation myocardial infarction; UA = unstable angina pectoris; UH = unheralded myocardial infarction.

View larger version (17K): [in a new window]   Figure 5 Spearman correlation of frequencies of circulating interferon (IFN)-+/CD3+ T cells and mRNA transcript levels of IFN- (A) and signal transducer and activator of transcription 4 (B). Area of the density ellipse represents the 95% confidence interval. RU = relative units; STAT = signal transducer and activator of transcription.