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J Am Coll Cardiol, 2004; 44:1578-1583, doi:10.1016/j.jacc.2004.06.073
© 2004 by the American College of Cardiology Foundation
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Proteomic analysis of plasma from patients during an acute coronary syndrome

Petra J. Mateos-Cáceres, PhD*, Antonio García-Méndez, PhD*, Antonio López Farré, PhD*, Carlos Macaya, MD, PhD*, Antonio Núñez, MD, PhD,*, Juan Gómez, MD, PhD*, Sergio Alonso-Orgaz, PhD*, Carolina Carrasco, PhD*, Marta Escribano Burgos, PhD*, Raimundo de Andrés, MD{dagger}, Juan J. Granizo, MD{ddagger}, Jerónimo Farré, MD,* and Luis A. Rico, MD, PhD*,*

* Cardiovascular Institute, Hospital Clínico San Carlos, Madrid, Spain
{dagger} Emergency Unit
{ddagger} Epidemiology Department, Cardiovascular Research Laboratory, Fundación Jiménez Díaz, Madrid, Spain



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  		Figure 1 (Left) Two-dimensional gel of plasma from a control volunteer using a pH range of 3 to 10. Four areas were analyzed (1 to 4) in each group of subjects. (Right) Representative bidimensional electrophoresis gel images of plasma from patients during acute myocardial infarction (AMI), unstable angina (UA), and age-matched control subjects using a pH range of 4 to 7. Albumin was identified in area 5 and was used to normalize total spot volume in each of the other areas.

 


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  		Figure 2 Representative pattern of alpha1-antitrypsin (AAT-1) in plasma from patients during acute myocardial infarction (AMI), unstable angina (UA), and age-matched control subjects. Images were obtained from the bidimensional electrophoresis gels using a pH range of 4 to 7. Numbers 1 to 7 were only used to identify each AAT isoform.

 


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  		Figure 3 Representative pattern of fibrinogen gamma chain in plasma from patients during acute myocardial infarction (AMI), unstable angina (UA), and age-matched control subjects. Images were obtained from the bidimensional electrophoresis gels using a pH range of 4 to 7. Numbers 1 to 3 were only used to identify each fibrinogen gamma chain isoform.

 


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  		Figure 4 Representative patterns of apolipoprotein A-I in plasma from patients during acute myocardial infarction (AMI), unstable angina (UA), and age-matched control subjects. Images were obtained from the bidimensional electrophoresis gels using a pH range of 4 to 7. Numbers 1 to 5 were only used to identify each apolipoprotein A-I isoform.

 




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Copyright © 2004 by the American College of Cardiology Foundation.