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Effect of granulocyte-macrophage colony-stimulating factor inducer on left ventricular remodeling after acute myocardial infarction

Division of Cardiology, Department of Medicine, Keio University School of Medicine, Tokyo, Japan

View larger version (29K): [in a new window]   Figure 1 (A) Representative hematoxylin-eosin-stained midventricular cross-sections of myocardial infarction rats treated with saline (MI/C) and myocardial infarction treated with romurtide (MI/Ro) on day 14. (B) Expansion index on day 14 in MI/C and MI/Ro (n = 4 per group). Values are mean ± SEM. *p < 0.05 versus MI/C.

View larger version (20K): [in a new window]   Figure 2 (A) Time course difference of peripheral leukocyte count among sham-operated rats (Sham), Sham treated with romurtide (Sham/Ro), myocardial infarction rats treated with saline (MI/C), and myocardial infarction treated with romurtide (MI/Ro) (n = 4 per group). (B) Time course difference of peripheral monocyte count among Sham, Sham/Ro, MI/C, and MI/Ro (n = 4 per group). Values are mean ± SEM. *p < 0.05 versus Sham; p < 0.05 versus MI/C.

View larger version (45K): [in a new window]   Figure 3 (A) Representative immunoblots showing transforming growth factor (TGF)-ß1 protein level in the infarcted and non-infarcted sites on days 3 and 14 among sham-operated rats (Sham), Sham treated with romurtide (Sham/Ro), myocardial infarction rats treated with saline (MI/C), and MI treated with romurtide (MI/Ro). (B) Quantified data of TGF-ß1 protein expression in the infarcted and non-infarcted sites among Sham, Sham/Ro, MI/C, and MI/Ro (n = 4 per group). Values are mean ± SEM. *p < 0.05 versus Sham; p < 0.05 versus Sham/Ro; p < 0.05 versus MI/C.

View larger version (51K): [in a new window]   Figure 4 Effects of romurtide administration on monocyte chemoattractant protein (MCP)-1, transforming growth factor (TGF)-ß1, and collagen type I and collagen type III messenger ribonucleic acid (mRNA) expression in the infarcted and non-infarcted sites. Quantitative analyses showing (A) MCP-1, (B) TGF-ß1, (C) collagen type I, and (D) collagen type III mRNA expression in the infarcted sites and (E) MCP-1, (F) TGF-ß1, (G) collagen type I, and (H) collagen type III mRNA expression in the non-infarcted sites with real-time reverse transcription polymerase chain reaction analyses among sham-operated rats (Sham), Sham treated with romurtide (Sham/Ro), myocardial infarction rats treated with saline (MI/C), and MI treated with romurtide (MI/Ro) (n = 5 per group). Values are mean ± SEM. *p < 0.05 versus Sham; p < 0.05 versus Sham/Ro; p < 0.05 versus MI/C. Continued on next page.

View larger version (71K): [in a new window]   Figure 5 (A) Results of immunohistochemical staining of the infarcted sites of the myocardial infarction rats treated with saline (MI/C) and MI treated with romurtide (MI/Ro), and of the left ventricle (LV) of the sham-operated rats (Sham) and Sham treated with romurtide (Sham/Ro) on day 7 with use of monoclonal anti-ED-1 and anti-monocyte chemoattractant protein-1 (anti-MCP-1)antibodies (magnification 400x). The ED-1-positive macrophages and MCP-1-positive cells are stained brown. (B) Mallory-Azan staining in the infarcted sites of the MI animals (MI/C and MI/Ro) and in the LV of the sham animals (Sham and Sham/Ro) on days 7 and 14 (magnification 400x). Fibrous tissue appears blue. (C) Collagen content in the infarcted and non-infarcted sites of MI/C and MI/Ro and in the LV of Sham and Sham/Ro (n = 4 per group). Values are mean ± SEM. *p < 0.05 versus Sham; p < 0.05 versus Sham/Ro; p < 0.05 versus MI/C.