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Comparison of human skeletal myoblasts and bone marrow-derived CD133⁺ progenitors for the repair of infarcted myocardium

Onnik Agbulut, PhD^*,*, Susanne Vandervelde^*, Nawwar Al Attar, MD^*, Jérôme Larghero, MD, Said Ghostine, MD, Bertrand Léobon, MD^||||, Estelle Robidel^*, Paolo Borsani, MD^*, Marc Le Lorc'h^¶¶, Alvine Bissery, PhD^||, Christine Chomienne, MD, PhD, Patrick Bruneval, MD, PhD^¶#, Jean-Pierre Marolleau, MD, Jean-Thomas Vilquin, PhD, Albert Hagège, MD, PhD, Jane-Lyse Samuel, PhD^* and Philippe Menasché, MD, PhD^**

^* Inserm U572, Hôpital Lariboisiére, Paris, France
Inserm U633, Hôpital Européen Georges Pompidou, Paris, France
Department of Cardiology, Hôpital Européen Georges Pompidou, Paris, France
^|| Clinical Investigation Center 9201, Hôpital Européen Georges Pompidou, Paris, France
^¶ Department of Pathology, Hôpital Européen Georges Pompidou, Paris, France
^# Inserm U430, Hôpital Européen Georges Pompidou, Paris, France
^** Department of Cardiovascular Surgery, Hôpital Européen Georges PompidouParis, France
Inserm E0003, Paris, France
Laboratory of Cell Therapy, Hôpital Saint-LouisParis, France
Inserm U582, Paris, France
^|||| Assistance Publique-Hôpitaux de Paris, Ecole de Chirurgie; Paris, France
^¶¶ Department of Cytogenetics, Hôpital Necker, Paris, France

View larger version (26K): [in a new window]   Figure 1 Functional effects of CD133+ and skeletal myoblast (SM) cell transplantation. (A) Individual values and mean percentage of change in left ventricular ejection fraction (LVEF) from baseline pretransplantation values in controls (n = 9; open triangles), CD133+-treated (n = 15; closed circles), and SM-treated (n = 8; closed triangles) hearts. (B) Representative tracings of pressure-volume occlusion loops. (C and D) Individual values and means of (C) maximal elastance (Emax) and (D) preload-recruitable stroke work (PRSW) in controls (n = 9), CD133+-grafted (n = 12), and SM-grafted (n = 7) hearts. Note the functional improvement in >25% of CD133+-grafted and SM-grafted animals.*p = 0.0015, p = 0.008, p = 0.01 vs. controls.

View larger version (120K): [in a new window]   Figure 2 Detection of human cells in grafted rat hearts. One month after skeletal myoblast (SM) grafting, immunofluorescence shows that many cells were positively stained for human lamins-A/C (A); some of them expressed (B) neonatal myosin heavy chain (MyHC) (arrows) and (C) adult-fast MyHC. (D) Detection via polymerase chain reaction of human cells in grafted hearts using a human-specific Alu primer.