Preservation of ischemic myocardial function and integrity with targeted cytoskeleton-specific immunoliposomes
Tala Khudairi, PhD* and
Ban-An Khaw, PhD*,*
* Center for Cardiovascular Targeting, Department of Pharmaceutical Sciences, Bouvé College of Health Sciences, Boston, Massachusetts, USA
Department of Biology, Northeastern University, Boston, Massachusetts, USA
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Figure 1 (A) A digital photograph of a representative nitro blue tetrazolium-stained heart slice (top left), a color-contrasted version to highlight the infarcted regions (top right), and an outline of the planimetered infarct area for infarct sizing (bottom). (B) Mean left ventricular developed pressures (LVDPs) (% pre-ischemic values) at 5 to 30 min of reperfusion after 25 min of global ischemia of hearts treated with cytoskeletal specific immunoliposomes (CSIL) at 1 min of ischemia (CSIL 1') (squares), PBS (open circles), or sham-instrumented hearts (solid circles). (C) Representative digital photographs of nitro blue tetrazolium-stained mid-slices of normal hearts (a) or hearts treated with CSIL 1' (b) or PBS (c).
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Figure 2 (A) Mean left ventricular developed pressure (LVDP) during reperfusion of hearts treated with cytoskeletal specific immunoliposomes (CSIL) at 5 min of ischemia (CSIL 5'), immunoglobulin G liposomes (IgG-L) at 5 min of ischemia (IgG-L 5'), phosphate buffered saline (PBS), or sham instrumentation. (B) Mean LVDP during reperfusion of hearts treated with CSIL or IgG-L at 10 min of ischemia (CSIL 10' and IgG-L 10'), PBS, or sham. (C) Mean LVDP during reperfusion of hearts treated with CSIL or IgG-L at 20 min of ischemia (CSIL 20' and IgG-L 20'), PBS, or sham. (A through C) Sham = solid circles; CSIL = squares; IgG-L = triangles; PBS = open circles. (D) Mean LVDP assessed at 20 to 30 min of reperfusion (plateau phase) in hearts treated with CSIL, IgG-L, or PBS.
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Figure 3 (A) Digital photographs of mid-slices of nitro blue tetrazolium-stained hearts treated with cytoskeletal specific immunoliposomes (CSIL) at 5 (a), 10 (b), and 20 (c) min of ischemia or immunoglobulin G liposomes (IgG-L) at 5 (d), 10 (e), and 20 (f) min of ischemia. (B) Mean infarct sizes are expressed as the percentage of right and left ventricles of hearts treated with CSIL, IgG-L, phosphate buffered saline (PBS), or sham instrumentation.
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Figure 4 Mean pixel sizes of mitochondria in normal hearts, those treated with cytoskeletal specific immunoliposomes (CSIL) and immunoglobulin G liposomes (IgG-L) at various times after initiation of ischemia, and placebo-treated hearts.
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