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Temporal patterns of gene expression after acute hindlimb ischemia in mice

insights into the genomic program for collateral vessel development

Cheol Whan Lee, MD^*, Eugenio Stabile, MD^*, Timothy Kinnaird, MD^*, Matie Shou, MD^*, Joseph M. Devaney, PhD^*, Stephen E. Epstein, MD^* and Mary Susan Burnett, PhD^*

^* Laboratory of Vascular Biology, Cardiovascular Research Institute, MedStar Research Institute, Washington Hospital Center, Washington, DC, USA
Research Center for Genetic Medicine, Children's National Medical Center, Washington, DC, USA

View larger version (16K): [in a new window]   Figure 1 Mouse hindlimb model. Schematic drawing of the femoral artery ligation sites. The femoral artery was ligated and cut immediately distal to the inguinal ligament and proximal to the popliteal bifurcation site. The entire adductor muscle was used for array studies and Western blotting.

View larger version (29K): [in a new window]   Figure 2 Expression profiles clustered according to similarity of temporal expression patterns, using k-means cluster analysis. Early induction includes upregulated genes whose peak expression occurred at 6 to 24 h after ligation. Mid-phase induction includes upregulated genes whose peak expression occurred at day 3. Late-phase induction includes upregulated genes whose peak expression occurred at days 7 to 14. Downregulation includes genes whose expression was downregulated (<0.5-fold). FAL = femoral artery ligation.

View larger version (30K): [in a new window]   Figure 3 Time course of hypoxia-inducible factor-1 (HIF-1) alpha expression in adductor and calf muscles after femoral artery ligation. C = control.

View larger version (18K): [in a new window]   Figure 4 Schematic summary of the time course of expression of selected groups of genes after femoral artery ligation. IL = interleukin; IP = interferon-gamma-inducible protein; MCP = monocyte chemoattractant protein; MIP = macrophage inflammatory protein; MMP = metalloelastase (metalloproteinase).