Unchanged G-protein-coupled receptor kinase activity in the aging human heart
Kirsten Leineweber, PhD*,
Stefan Klapproth, BS*,
Anja Beilfuß*,
Rolf-Edgar Silber, MD ,
Gerd Heusch, MD, FACC*,
Thomas Philipp, MD* and
Otto-Erich Brodde, PhD*,*
* Departments of Pathophysiology and Nephrology, University of Essen School of Medicine, Essen, Germany
Clinic for Cardiothoracic Surgery, Martin-Luther-University of Halle-Wittenberg, Halle, Germany
View larger version (46K):
[in a new window]
|
Figure 1 (A) Typical autoradiopraph of light-dependent in vitro phosphorylation of rhodopsin (32P-ROS) by atrial cytosolic (CYT) and membranous (MEM) GRK obtained from a child (3-year-old male; NYHA class 0) and an elderly patient (60-year-old male; NYHA class I). (B) Bar graph showing cytosolic and membranous GRK activity in right atrial appendages obtained from 10 children (5 males and 5 females; mean age 6 ± 2 years [range 3 months to 20 years]; NYHA class 0.9 ± 0.3) and eight elderly patients (4 males and 4 females; mean age 68 ± 3 years [range 59 to 80]; NYHA class 1.9 ± 0.4). Activities were calculated as 32P incorporated in rhodopsin in cpm x 103 per mg protein. Data are given as the mean value ± SEM.
|
|
View larger version (46K):
[in a new window]
|
Figure 2 (A) Typical autoradiopraph of light-dependent in vitro phosphorylation of rhodopsin (32P-ROS) by cytosolic (CYT) and membranous (MEM) GRK obtained from the RA from a non-failing (3-year-old male donor; NYHA class 0) and failing (61-year-old male recipient; NYHA class IV) heart. (B) Bar graph showing cytosolic and membranous GRK activity in right atrial appendages obtained from four non-failing (mean age 30 ± 12 years [range 3 to 52]; 3 males and 1 female; NYHA class 0) and four failing (mean age 51 ± 4 years [range 44 to 63]; 4 males; NYHA class IV) hearts. Activities were calculated as 32P incorporated in rhodopsin in cpm/mg protein. Data are given as the mean value ± SEM. *p = 0.0381 vs. non-failing heart.
|
|
View larger version (13K):
[in a new window]
|
Figure 3 Adenylyl cyclase activity in right atrial appendages obtained from six children (4 males and 2 females; mean age 13 ± 3 years [range 4 to 21]; open columns) and nine elderly patients (5 males and 4 females; mean age 66 ± 3 years [range 52 to 78]; solid columns). Data are given as the mean value ± SEM. *p < 0.05 vs. children. B = basal; GTP = 10 µmol/l guanosine triphosphate; ISO = 10 µmol/l isoprenaline; FOR = 10 µmol/l forskolin.
|
|
|
