HOME SUBSCRIPTIONS CURRENT ISSUE PAST ISSUES CARDIOSOURCE SEARCH HELP FEEDBACK		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bowles, N. E. Articles by Towbin, J. A. Search for Related Content PubMed PubMed Citation Articles by Bowles, N. E. Articles by Towbin, J. A.

Detection of viruses in myocardial tissues by polymerase chain reaction

evidence of adenovirus as a common cause of myocarditis in children and adults

Neil E. Bowles, PhD^*, Jiyuan Ni, MD^*, Debra L. Kearney, MD^*, Matthias Pauschinger, MD, Heinz-Peter Schultheiss, MD, Robert McCarthy, MD^¶, Joshua Hare, MD, FACC, J. Timothy Bricker, MD, FACC^*, Karla R. Bowles, PhD^* and Jeffrey A. Towbin, MD, FACC^*||,*

^* Departments of Pediatrics, Houston, Texas, USA
Pathology, Houston, Texas, USA
^|| Molecular and Human Genetics, Houston, Texas, USA
^¶ Cardiovascular Sciences, Baylor College of Medicine, Houston, Texas, USA
Medical Clinic II, University Hospital Benjamin Franklin, Berlin, Germany
Department of Internal Medicine, Division of Cardiology, The Johns Hopkins Hospital, Baltimore, Maryland, USA

View larger version (29K): [in a new window]   Figure 1 (Left) A nine-month-old (TT) presented with a one-day history of fever, irritability, poor feeding, respiratory distress, and poor ventricular function by echocardiography. Death ensued within four hours of presenting to the emergency room. Formalin-fixed cardiac tissue (TTRV1) was positive for adenovirus by polymerase chain reaction (PCR), as demonstrated by the 308 bp PCR product—the same size seen with the adenovirus type 5 control. The 135 bp band is the k-ras PCR product. Note that Patient ALH’s right ventricular endomyocardial biopsy was negative for the adenoviral PCR product but amplifies the 135 bp k-ras product. (Right) Southern blot and hybridization with a radiolabeled adenovirus probe.

View larger version (48K): [in a new window]   Figure 2 Number of myocarditis patients by age group, positive for viruses by polymerase chain reaction. CMV = cytomegalovirus; EBV = Epstein-Barr virus; HSV = herpes simplex virus; RSV = respiratory syncytial virus.

View larger version (25K): [in a new window]   Figure 3 Number of dilated cardiomyopathy patients by age group, positive for viruses by polymerase chain reaction. Black bars = adenovirus; lined bars = enterovirus.