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The T^-786C and Glu298Asp polymorphisms of the endothelial nitric oxide gene affect the forearm blood flow responses of Caucasian hypertensive patients

Gian Paolo Rossi, MD, FACC, FAHA^*,*, Stefano Taddei, MD, Agostino Virdis, MD, Martina Cavallin, BiolD^*, Lorenzo Ghiadoni, MD, Stefania Favilla, BiolD, Daniele Versari, Isabella Sudano, MD, Achille C. Pessina, MD, PhD^* and Antonio Salvetti, MD

^* Department of Clinical and Experimental Medicine, Clinica Medica 4, University of Padova, Padova, Italy
Department of Internal Medicine, University of Pisa Pisa, Italy

View larger version (20K): [in a new window]   Figure 1 Representative results of melting curve analysis of the polymerase chain reaction products from subjects who were genotyped for the T-786C (upper) and the Glu298Asp (lower) polymorphism. An unequivocal identification of the different genotypes was consistently accomplished with a 100% accuracy versus sequencing (see text for details).

View larger version (21K): [in a new window]   Figure 2 Changes in age-adjusted forearm blood flow responses to an intrabrachial artery infusion of the endothelial-dependent vasodilator acetylcholine in the normotensive (NT) subjects (A and C) and in the uncomplicated patients with mild-to-moderate essential hypertension (PH) (B and D) divided according to the T-786C (A and B) and Glu298Asp (C and D) endothelial nitric oxide synthase (eNOS) genotypes. A significantly blunted response was seen in the PH patients as compared with the NT subjects. In addition, an enhanced response was seen in the PH patients who were TT homozygous (n = 30) at the T-786C eNOS genotype, as compared with the TC (n = 69) and CC (n = 38) genotypes. At variance, no significant differences were seen between the PH patients and NT subjects classified according to the Glu298Asp (G894T) eNOS genotype. Mean ± SEM. *p < 0.01; **p < 0.001 by analysis of variance and post-hoc Bonferroni test of TT versus the other genotypes.