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Transplantation of cells for cardiac repair

Rutger J. Hassink, MD^*,*, Aart Brutel de la Rivière, MD, PhD^*, Christine L. Mummery, PhD and Pieter A. Doevendans, MD, PhD

^* University Medical Center, Heart Lung Center, Department of Cardio-Thoracic Surgery, Utrecht, The Netherlands
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht, The Netherlands
Interuniversity Cardiology Institute of the Netherlands, Utrecht, The Netherlands

View larger version (80K): [in a new window]   Figure 1 (A) Mouse embryonic stem (ES) cells (white arrows) cultured on mouse embryonic fibroblasts (black arrows). (B) Mouse ES cells cultivated in aggregates in suspension to form floating embryoid bodies. (C) Mouse ES cells differentiated into beating muscle (white arrow) on visceral endoderm (black arrow).

View larger version (81K): [in a new window]   Figure 2 A-actinin immunohistochemical staining, dilution 1:400. Secondary antibody: goat anti-mouse IgG, dilution 1:250.