Nitric oxide production by neutrophils obtained from patients during acute coronary syndromes: expression of the nitric oxide synthase isoforms
Lourdes Sánchez de Miguel, PhD,
M. a Mar Arriero, PhD*,
Jerónimo Farré, MD, PhD ,
Petra Jiménez, PhD,
Antonio García-Méndez, PhD,
Trinidad de Frutos, PhD,
Ana Jiménez, PhD,
Rosa García, PhD,
Fernando Cabestrero, MD,
Juan Gómez, MD ,
Raimundo de Andrés, MD,
Mercedes Montón, PhD*,
Edita Martín, RN,
Luz M. De la Calle-Lombana, RN,
Luis Rico, MD ,
José Romero, MD and
Antonio López-Farré, PhD*,*
* Cardiovascular Research and Hypertension Laboratory, Fundación Jiménez Díaz, Madrid, Spain
Cardiology Department, Fundación Jiménez Díaz, Madrid, Spain
Cardiovascular Research Hypertension Laboratory, Emergency Department, Fundación Jiménez Díaz, Madrid, Spain
Cardiovascular Research Hypertension Laboratory, Internal Medicine Department, Fundación Jiménez Díaz, Madrid, Spain
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Figure 1 (Top) Representative Western blot demonstrating endothelial nitric oxide synthase (eNOS) expression in human neutrophils obtained from healthy volunteers (control) and patients during acute myocardial infarction (AMI) and unstable angina (UA). (Bottom) Bar graph showing the densitometric analysis of the Western blot. Results are presented as mean ± SEM. *p < 0.05 with respect to neutrophils obtained from healthy donors.
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Figure 2 (Top) Representative Western blot demonstrating inducible nitric oxide synthase (iNOS) expression in human neutrophils obtained from healthy volunteers (control) and patientes during acute myocardial infarction (AMI) and unstable angina (UA). (Bottom) Bar graph showing the densitometric analysis of the Western blot. Results are presented as mean ± SEM. *p < 0.05 with respect to neutrophils obtained from healthy donors. **p < 0.05 with respect to neutrophils from UA patients.
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Figure 3 Cyclic guanosine monophosphate (cGMP) levels in platelets and platelets plus neutrophils obtained from healthy volunteers and patients during acute coronary syndromes. Determination of cGMP levels were performed in platelets alone (PLT) or coincubated with neutrophils (PMN). Platelets and neutrophils were obtained from peripheral blood of healthy volunteers (control) and patients during unstable angina (UA) and during acute myocardial infaction (AMI). Results are represented as mean ± SEM. *p < 0.05 with respect to the corresponding platelets alone.
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Figure 4 Bar graph showing superoxide anion production by neutrophils. Neutrophils were obtained from control subjects (control), acute myocardial infartion (AMI) and unstable angina (UA) patients. Results are presented as mean ± SEM. *p < 0.05 with respect to neutrophils obtained from healthy donors.
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Figure 5 (Top) Representative Western blot demonstrating the presence of nitrotyrosilated proteins in neutrophils from healthy volunteers (control) and patients during acute myocardial infarction (AMI) and unstable angina (UA). (Bottom) Bar graph showing the densitometric analysis of the nitrotyrosilated proteins with apparent molecular weights of 16, 24, 84 KDa that was observed in the Western blot. Results are mean ± SEM. Asterisk = p < 0.05 with respect to neutrophils from healthy donor; star = p < 0.05 with respect to neutrophils from UA patients. White bars = 16 KDa; grey bars = 24 KDa; black bars = 84 KDa.
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