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Myocardial creatine kinase expression after left ventricular assist device support

Soon J. Park, MD^*, Jianyi Zhang, MD, PhD^,*, Yun Ye, MD, MS, Sofia Ormaza, RN, MS, Peihua Liang, MS, Alan J. Bank, MD, Leslie W. Miller, MD and Robert J. Bache, MD

^* Department of Surgery, University of Minnesota, Minneapolis, Minnesota, USA
Cardiovascular Division, Department of Medicine, University of Minnesota, Minneapolis, Minnesota, USA

View larger version (26K): [in a new window]   Figure 1 Densitometric intensities for protein bands from Western blots of creatine kinase (CK)-M, CK-B and creatine kinase-mitochondrial (CK-Mt) isoforms normalized to beta-actin. A through F represents six individual patients before and after left ventricular assist device (LVAD) implantation. The protein levels of CK-M and CK-Mt isoforms were increased after LVAD implantation while CK-B and beta-actin were unchanged.

View larger version (19K): [in a new window]   Figure 2 Densitometric intensities for protein bands from Western blots of creatine kinase (CK)-M (A), CK-B (B) and creatine kinase-mitochondrial (CK-Mt) (C) isoforms normalized to beta-actin. The ratio of CK-M and CK-Mt to beta-actin increased significantly after hemodynamic unloading provided by the left ventricular assist device (LVAD). Values are mean ± SEM. *p < 0.05 versus pre-LVAD. Black bars = pre-LVAD; white bar = post-LVAD.

View larger version (41K): [in a new window]   Figure 3 CK-M protein normalized to beta-actin before and after left ventricular assist device (LVAD) implantation in patients with heart failure (A). Creatine kinase-mitochondrial (CK-Mt) normalized to beta-actin before and after LVAD implantation in patients with heart failure (B). CK-B normalized to beta-actin before and after LVAD implantation in patients with heart failure (C). Total CK activity per mg cardiac protein before and after LVAD implantation in patients with heart failure (D). Data are from seven hearts in which both pre- and post-LVAD specimens were available. Each symbol represents the specimens from the same heart.