HOME SUBSCRIPTIONS CURRENT ISSUE PAST ISSUES CARDIOSOURCE SEARCH HELP FEEDBACK		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Durand, E. Articles by Lafont, A. Search for Related Content PubMed PubMed Citation Articles by Durand, E. Articles by Lafont, A.

Time courses of apoptosis and cell proliferation and their relationship to arterial remodeling and restenosis after angioplasty in an atherosclerotic rabbit model

Eric Durand, MD^*, Ziad Mallat, MD, PhD, Faouzi Addad, MD^*, Françoise Vilde, MD^*, Michel Desnos, MD^*, Claude Guérot, MD^*, Alain Tedgui, PhD and Antoine Lafont, MD, PhD^*,*

^* INSERM EMI-U 00-16 Paris, France
INSERM U 541, Paris, France

View larger version (100K): [in a new window]   Figure 1 Rabbit femoral artery 14 days after balloon angioplasty. (A) Macrophages (brown) revealed by monoclonal anti-rabbit macrophage (RAM11). (B) Smooth muscle cells (brown) in the neointima and media, revealed by a monoclonal antibody against alpha-smooth muscle actin. (C) Apoptotic nuclei (purple, arrowheads) in the neointima and media, using T4 deoxyribonucleic acid ligase. (D) Absence of detection of ribonucleic acid splicing, using monoclonal antibody against SC-35. Magnification x40 (A to D). Counterstaining with hematoxylin (A and B).

View larger version (82K): [in a new window]   Figure 2 In situ detection of apoptosis in the rabbit atherosclerotic femoral artery 14 days after balloon angioplasty. (A) Monoclonal antibody against poly (ADP-ribose) polymerse-1 (red). (B) T4 deoxyribonucleic acid (DNA) ligase (purple). (C) Single-stranded DNA (red). (D) Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (brown). Magnification x40 (A–D). Counterstaining with hematoxylin (D).

View larger version (21K): [in a new window]   Figure 3 Bar graph representing apoptosis (A) and cell proliferation (B) at 2 h (n = 4) and 3 (n = 4), 7 (n = 8), 14 (n = 8), 21 (n = 8) and 28 (n = 8) days after angioplasty in nondilated arteries (n = 8), the neointima (solid bar), the media (hatched bar) and the adventitia (open bar). Results are expressed as the mean value ± SD.

View larger version (17K): [in a new window]   Figure 4 Scattergrams of apoptosis in the neointima (A and C) and media (B and D) versus restenosis (A and B) and arterial remodeling (C and D). Correlations were evaluated by the Spearman rank correlation test.