This Article Abstract Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Takajo, Y. Articles by Imaizumi, T. Search for Related Content PubMed PubMed Citation Articles by Takajo, Y. Articles by Imaizumi, T.

Augmented oxidative stress of platelets in chronic smokers

Mechanisms of impaired platelet-derived nitric oxide bioactivity and augmented platelet aggregability

^a Department of Internal Medicine III and Cardiovascular Research Institute, Kurume University School of Medicine, Kurume, Japan

View larger version (35K): [in a new window]   Figure 1 Collagen-induced platelet-derived electrical currents (A) and intraplatelet cyclic guanosine 3',5'-monophosphate (cGMP) levels (B) before and after vitamin C administration in nonsmokers (n = 10) and smokers (n = 11). Collagen-induced platelet aggregation before (C) and after (D) vitamin C administration in nonsmokers (n = 10) and smokers (n = 11). Open bars = nonsmokers; solid bars = smokers; NS = nonsignificant.

View larger version (29K): [in a new window]   Figure 2 Time courses of plasma (A) and intraplatelet (B) levels of vitamin C in nonsmokers (n = 10; open circles) and smokers (n = 11; solid circles). *p < 0.05 indicates the difference between nonsmokers and smokers before vitamin C administration. (C) Intraplatelet reduced glutathione (GSH) levels before and after vitamin C administration in nonsmokers (n = 10) and smokers (n = 11). NS = nonsignificant.

View larger version (35K): [in a new window]   Figure 3 Consumption of intraplatelet vitamin C (A) and reduced glutathione (GSH) (B) by platelet aggregation before and after vitamin C administration in nonsmokers (n = 10) and smokers (n = 11). Correlation between the collagen-induced platelet-derived electrical current and consumption of intraplatelet GSH by platelet aggregation before and after vitamin C administration in (C) nonsmokers (n = 10) and (D) smokers (n = 11). NS = nonsignificant.

View larger version (21K): [in a new window]   Figure 4 (A–C) Representative histograms of intraplatelet nitrotyrosine production in a nonsmoker and a smoker. In the smoker before vitamin C administration, the fluorescent peak of nitrotyrosine in platelets stimulated by collagen (3 µg/ml) (open area) shifted to the right from fluorescent peaks of unstimulated platelets (solid area). In the smoker after vitamin C administration, the histograms were identical to those of the nonsmoker. (D) Intraplatelet nitrotyrosine production by collagen-induced platelet aggregation in nonsmokers (n = 8) and in smokers (n = 8).