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Physical training in patients with chronic heart failure enhances the expression of genes encoding antioxidative enzymes

Pierre V. Ennezat, MD^* , Slawomir L. Malendowicz, MD^* , Marco Testa, MD, PhD^*, Paolo C. Colombo, MD^*, Alain Cohen-Solal, MD, Todd Evans, PhD and Thierry H. LeJemtel, MD^*

^* Department of Medicine, Division of Cardiology, Albert Einstein College of Medicine, Bronx, New York, USA
Department of Developmental and Molecular Biology, the Albert Einstein College of Medicine, Bronx, New York, USA
Division of Cardiology, Beaujon Hospital, Clichy, France

View larger version (71K): [in a new window]   Figure 1 Shown are representative autoradiographs used to measure the relative abundance of transcripts in RNA isolated from vastus lateralis muscle at baseline and after 12 weeks of training. Reverse transcription reactions included either 0.25 µg (1x) or 0.5 µg (2x) total RNA. Polymerase chain reaction conditions were chosen to ensure that product derived from the 2x sample was doubled compared with the 1x sample, demonstrating that the assays are at least semiquantitative. Aliquots of the same reverse transcription reaction for each patient were used for each polymerase chain reaction using primers specific to the various gene products. Cu/Zn SOD = copper zinc superoxide dismutase; eNOS = endothelial nitric oxide synthase; GSH-Px = glutathione peroxidase; VWF = Von Willebrand factor.

View larger version (10K): [in a new window]   Figure 2 Transcript levels of the shear-stress responsive genes endothelial nitric oxide synthase (eNOS), angiotensin-converting enzyme (ACE) and prostacyclin synthase (PGI2S) are unaltered in the skeletal vasculature of patients with chronic heart failure after 12 weeks of physical training. Shown in the graph, transcript levels are normalized to those from the Von Willebrand factor (VWF) gene to control for biopsy-dependent differences in vasculature. Open bars = pretraining; solid bars = posttraining. Data are presented mean ± SEM. Note that the data allow quantitative comparisons pre- and posttraining for each individual gene but do not analyze relative expression of the various genes to each other.

View larger version (10K): [in a new window]   Figure 3 Transcript levels of genes encoding antioxidant enzymes copper zinc superoxide dismutase (Cu/Zn SOD) and glutathione peroxidase (GSH-Px) are increased approximately 100% after 12 weeks of physical training. The Cu/Zn SOD and GSH-Px transcript levels were determined relative to Von Willebrand factor (VWF) complementary DNA in the same preparation of reverse transcribed RNA. Open bars = pretraining; solid bars = posttraining. Data are presented mean ± SEM. *p < 0.05 versus pretraining.