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Transendocardial delivery of autologous bone marrow enhances collateral perfusion and regional function in pigs with chronic experimental myocardial ischemia

Shmuel Fuchs, MD^*, Richard Baffour, PhD^*, Yi Fu Zhou, MD^*, Matie Shou, MD^*, Anthony Pierre, BSc^*, Fermin O. Tio, MD, Neil J. Weissman, MD^*, Martin B. Leon, MD, Stephen E. Epstein, MD^* and Ran Kornowski, MD^*

^* the Cardiovascular Research Institute, Washington, DC, USA
Biomedical Research Foundation of South Texas Inc., San-Antonio, Texas, USA
Cardiovascular Research Foundation, Lenox Hill Hospital, New York, New York, USA

View larger version (27K): [in a new window]   Figure 1 (A) Effects of conditioned medium on PAEC proliferation. Pig aortic endothelial cells were seeded, in triplicate, in 96 well plates for one day; subsequently, the indicated volumes of conditioned medium from pooled samples or control medium were added (with comparable volume of PAEC medium removed). After four days, PAEC, cultured with conditioned medium or control medium, were harvested and counted. (B) Effects of conditioned medium on tritiated thymidine uptake by PAEC. After two days, 1 µCi tritiated thymidine was added to each well, and 48 h later, DNA in PAEC was harvested, and radioactivity was counted. (C and D) Changes in bone marrow cell conditioned medium of VEGF (C) and MCP-1 (D) concentrations at one to four weeks, assayed by ELISA. MCP-1 = macrophage chemoattractant protein-1; PAEC = pig aortic endothelial cells; VEGF = vascular endothelial growth factor.

View larger version (80K): [in a new window]   Figure 2 Myocardial injection site stained for CD-34 (left) and hematoxylin and eosin (right) at three days after transendocardial injection of ABM. Note strong staining for CD-34 in 4% to 6% of the cells.