Cardiocyte cytoskeleton in patients with left ventricular pressure overload hypertrophy
Michael R. Zile, MD, FACC*,
G. Randall Green, MD ,
Gregg T. Schuyler, MD, PhD, FACC*,
Gerard P. Aurigemma, MD, FACC ,
D. Craig Miller, MD, FACC and
George Cooper, IV, MD*
* Gazes Cardiac Research Institute, Medical University of South Carolina and the Department of Veterans Affairs Medical Center, Charleston, South Carolina, USA
Department of Cardiovascular and Thoracic Surgery, Stanford University School of Medicine, Stanford, California, USA
Cardiology Division of the University of Massachusetts Medical Center, Worcester, Massachusetts, USA
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Figure 1 Left ventricular function in control patients and in patients with AS. (Both panels) The solid and parallel dashed lines define the relationship between midwall fractional shortening and mean systolic stress, as well as its 95% prediction interval, and calculated using a least squares linear regression analysis for 84 patients found not to have cardiac pathology. The shortening-stress relationship was characterized as noted in the "Methods" section identically for both the 84 patients used to generate the regression analysis and for the study patients having AS. (Panel A) Individual values for the 15 patients in this study. (Panel B) Summary data for these same patients grouped by functional status. AS = aortic stenosis.
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Figure 2 Representative and summarized myocardial tubulin data for normal patients and patients with AS. (Top) Immunoblot prepared from left ventricular biopsies from one patient from each of the four study groups defined in Figure 1. For comparison of the ratio of free heterodimer (lanes #1) to polymerized microtubule (lanes #2) tubulin in the four biopsies, an equal amount of tubulin protein was loaded for each of the free tubulin lanes, and an equal volume of the polymerized tubulin (microtubule) fraction was loaded for each of the polymerized tubulin lanes. (Bottom) Summary semiquantitative tubulin data for all of the patients in the four groups defined in Figure 1; the values were obtained from immunoblots wherein known quantities of concurrently run pure beta-tubulin were used as a standard, and the integrated optical density of the standards was compared with that of the unknown samples. Statistical analysis by one factor analysis of variance, which showed a significant (p < 0.05) F-ratio, was followed by a Fisher protected least significant difference post-hoc test. *p < 0.05 for difference from the value for the Control group. AS = aortic stenosis.
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Figure 3 The relationship of left ventricle contractile function to left ventricle microtubule protein for all of the patients in the four groups defined in Figure 1. AS = aortic stenosis.
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