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Evaluation of viral infection in the myocardium of patients with idiopathic dilated cardiomyopathy

^a Third Division, Department of Internal Medicine, Osaka Medical College, Takatsuki, Japan
^b Central Clinical Laboratory, Osaka Medical College, Takatsuki, Japan
^c Shonan Kamakura General Hospital, Heart Center, Kamakura, Japan

View larger version (40K): [in a new window]   Figure 1 Representative Southern blot hybridization analysis of enteroviral polymerase chain reaction products. Strand-specific enteroviral RNA detection in the myocardium from four different patients is shown. In lanes 3, 5, 7 and 9, RT-PCR was performed with 3' enteroviral primer for RT. The signals seen in lanes 5 and 9 correspond to enteroviral plus-strand cDNA. In lanes 4, 6, 8 and 10, RT-PCR was carried out with 5' enteroviral primer for RT. The signal seen in lane 6 corresponds to enteroviral minus-strand cDNA. In summary, Patient 1 (lanes 5 and 6) shows active enteroviral replication (minus-strand detectable). Patient 9 (lanes 9 and 10) shows latent virus persistence (not minus-strand detectable). Patient 11 (lanes 3 and 4) and Patient 18 (lanes 7 and 8) are negative for enteroviral genome. Lane 1 = positive control of coxsackie B1 cloned DNA. Lane 2 = negative control. Patients No. 1, 9, 11 and 18; see Table 1. cDNA = complementary DNA; RT-PCR = reverse transcription polymerase chain reaction.

View larger version (22K): [in a new window]   Figure 2 Alignment of nucleotide sequences of enterovirus polymerase chain reaction products derived from the myocardium of patients with idiopathic dilated cardiomyopathy (Patients No. 1, 2, 3, 6 and 7; see Table 1). Published sequences of coxsackievirus B3 (strain Nancy), coxsackievirus B3 (clinical isolate) and coxsackievirus B4 (strain J.V.B.) are shown in full, and any differences between these are indicated below by the presence of that base. A = adenine; C = cytocine; G = guanine; T = thymine; CVB3 = coxsackievirus B3 (strain Nancy); CVB4 = coxsackievirus B4 (strain J.V.B.); wCVB3 = coxsackievirus B3 (clinical isolate).