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Enhanced phospholipase C activity in the cultured skin fibroblast obtained from patients with coronary spastic angina: possible role for enhanced vasoconstrictor response

Ken Okumura, MD^*, Tomohiro Osanai, MD^*, Takuo Kosugi, MD^*, Hiroyuki Hanada, MD^*, Hiroshi Ishizaka, MD^*, Tomohisa Fukushi, MD^*, Takaatsu Kamada, MD^*, Takeshi Miura, MD^*, Toru Hatayama, MD^*, Takao Nakano, MD^*, Yasuhiro Fujino, MD^* and Yoshimi Homma, PhD

^* Second Department of Internal Medicine, Hirosaki University School of Medicine, Hirosaki, Japan
Department of Biomolecular Science, Fukushima Medical College, Fukushima, Japan

View larger version (20K): [in a new window]   Figure 1 Mean phospholipase C activity of the three groups. See text for details.

View larger version (19K): [in a new window]   Figure 2 Relationship between the average basal coronary artery tone and PLC activity. Open circles = control subjects without coronary spasm; solid circles = patients with CSA. See text for details.

View larger version (37K): [in a new window]   Figure 3 Relations between the maximal constrictor response to ACh from the baseline diameter and PLC activity (A), between the maximal constrictor response normalized by ISDN and PLC activity (B), between the average response to ACh from the baseline diameter and PLC activity (C) and between the average response normalized by ISDN and PLC activity (D). Open circles = control subjects without coronary spasm; solid circles = patients with CSA. See text for details.

View larger version (21K): [in a new window]   Figure 4 Western blot analysis of the membrane fraction, demonstrating the phospholipase C-1 isoenzyme stained as 70- and 85-kd (kDa) bands. Lanes 1 and 2 = control subjects; lanes 3 and 4 = patients with CSA.