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Signaling and expression for mitochondrial membrane proteins during left ventricular remodeling and contractile failure after myocardial infarction

Xue-Han Ning, MD^{* c}, Jianyi Zhang, MD, PhD ^c, Jingbo Liu, MD, PhD ^c, Yun Ye, MD ^c, Shi-Han Chen, PhD^{* c}, Arthur H. L. From, MD ^c, Robert J. Bache, MD, FACC ^c and Michael A. Portman, MD, FACC^{* c}

^* Cardiology Division, Department of Pediatrics, University of Washington, Seattle, Washington, USA
Department of Medicine, University of Minnesota Health Sciences Center, Minneapolis, Minnesota, USA
^c Department of Veterans Affairs Medical Center, Minneapolis, Minnesota, USA. %

View larger version (53K): [in a new window]   Figure 1 A representative Northern blot. Each lane represents a different heart. Lanes were loaded with 15 µg total RNA from ventricular myocardium and probed specifically for, ß-F1-ATPase, ANT-1 and G3PDH. Samples were taken from the normal heart (N), the heart with postinfarction left ventricular remodeling (R) without failure and the heart with postinfarction left ventricular failure (F) (see text). ANT = adenine nucleotide translocator.

View larger version (31K): [in a new window]   Figure 2 Steady state mRNA levels. All transcript levels are relative to the G3PDH band intensity and normalized to the lane of the normal heart. Groups are N, normal; LVR, left ventricular remodeling and CHF, congestive heart failure. The data indicate that a significant decrease in these transcript levels occurs in CHF. ANT = adenine nucleotide translocator.

View larger version (39K): [in a new window]   Figure 3 Representative Western blots show relative amounts of mitochondrial ANT and ß-F1-ATPase in left ventricular myocardium in three different states (N, F, and R). Each lane represents a different heart. Substantial decreases in both mitochondrial proteins occur with heart failure (F). 43 kD protein band serves as a marker. The same amount of total protein (100 µg) was run in parallel lanes on a SDS gel for standardization purpose. ANT = adenine nucleotide translocator; F = heart with postinfarction left ventricular failure; N = normal heart; R = heart with postinfarction left ventricular remodeling.

View larger version (35K): [in a new window]   Figure 4 Densitometric intensities for protein bands from Western blots are normalized to the lane of the normal heart. The data indicate that a significant decrease in protein expression for ANT and ß-F1-ATPase occurs in CHF. ANT = adenine nucleotide translocator; CHF = congestive heart failure; LVR = left ventricular remodeling; N = normal heart.