Abnormal cardiac function in the streptozotocin-induced, non–insulin-dependent diabetic rat
Noninvasive assessment with Doppler echocardiography and contribution of the nitric oxide pathway
Ian I. Joffe, MDa,
Kerry E. Travers, BAa,
Cynthia L. Perreault-Micale, PhDa,
Thomas Hampton, PhDa,
Sarah E. Katz, BAa,
James P. Morgan, MD, PhD, FACCa and
Pamela S. Douglas, MD, FACCa
a Charles A. Dana Research Institute and the Harvard-Thorndike Laboratory, Cardiovascular Division, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA
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Figure 1 Exhaled NO versus diastolic filling pattern. Mixed expired NO levels were strongly correlated with a greater atrial contribution to diastolic filling (reduced E/A ratio) in all animals. Circles = diabetic animals; squares = control animals.
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Figure 2 Diastolic pressure–volume relation. The LV diastolic pressure was measured 1 to 2 min after each increment of balloon volume. To achieve comparable loading conditions (balloon volumes) in hearts of different sizes, the LV diastolic pressure was plotted versus vol/volmax. The diabetic pressure–volume relation was shifted to the left, with a significant change in the shape of the curve, indicating increased chamber stiffness. Line with triangles = diabetic animals; line with squares = control animals.
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Figure 3 Relative ecNos expression. A representative immunoblot of ecNOS in the LVs of control (Con) and diabetic (Diab) rats. The last lane contains a human endothelial cell lysate (Human endo) as a positive control. Note the apparent slight differences in molecular weight between the rat and human ecNOS isoforms. Quantification of band densities indicated no differences in the relative levels of ecNOS between control and diabetic rats’ LVs.
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