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The detection of viral genomes by polymerase chain reaction in the myocardium of pediatric patients with advanced HIV disease

Neil E. Bowles, PhD^* , Debra L. Kearney, MD , Jiyuan Ni, MD^* , Antonio R. Perez-Atayde, MD^||, Mark W. Kline, MD , J. Timothy Bricker, MD, FACC^* , Nancy A. Ayres, MD, FACC^* , Steven E. Lipshultz, MD^¶,2, William T. Shearer, MD, PhD and Jeffrey A. Towbin, MD, FACC^{* #,1 2}

^* Section of Cardiology, Baylor College of Medicine, Houston, Texas, USA
Section of Allergy and Immunology, Baylor College of Medicine, Houston, Texas, USA
Department of Pediatrics, Baylor College of Medicine, Houston, Texas, USA
Departments of Pediatrics and Pathology, Baylor College of Medicine, Houston, Texas, USA
^|| Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA
^¶ Division of Pediatric Cardiology, University of Rochester Medical Center, Rochester, New York, USA
^# Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA

View larger version (55K): [in a new window]   Figure 1 The detection of adenoviral genomic DNA by PCR using the primers designed to the hexon region. The products were detected by ethidium bromide staining of the 2% agarose gel. A 1-kb size ladder (size marker) is shown in the first lane. Adenovirus positive control PCR is seen as a 308-bp amplimer in the second lane. In the subsequent lanes are samples A92–106, A93–29, A83–90, A87–85 and A92–98. Note that samples A92–106 (case 26, Table 2) and A92–98 (case 2, Table 2) have bands that correspond in size to the positive control.