Cardiomyocytes from hearts with left ventricular dysfunction after ischemia-reperfusion do not manifest contractile abnormalities
Y. Chandrashekhar, MDa,
Arun J. Prahash, MB, BSa,
Soma Sen, MDa,
Sudhir Gupta, PhDa and
Inder S. Anand, DPhil, FRCP, (Oxon), FACCa
a Division of Cardiology, Veterans Affairs Medical Center and the University of Minnesota Medical School, Minneapolis, Minnesota, USA
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Figure 1 Dose-response curves for isolated cardiomyocyte shortening in response to increasing extracellular calcium. Normal cells imply cells isolated from normal hearts not subjected to prolonged buffer perfusion, unlike in the other two (stunning and control) groups. Cell shortening has been normalized to resting cell length.
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Figure 2 Changes in intracellular calcium in cells from control and stunned hearts, measured by fura-2 AM, during stimulation with varying degrees of extracellular calcium.
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Figure 3 Scatterplot of change in cell shortening versus change in intracellular calcium during stimulation with varying degrees of extracellular calcium. The slope of this line would represent shortening per unit change in intracellular calcium, a measure of calcium sensitivity.
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Figure 4 Change in cardiomyocyte shortening in face of increased external viscous loading. Cell shortening has been normalized to resting cell length.
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