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Exercise intolerance in patients with chronic heart failure and increased expression of inducible nitric oxide synthase in the skeletal muscle

^a Heart Center, Division of Cardiology, University of Leipzig, Leipzig, Germany

View larger version (65K): [in a new window]   Figure 1 Semi-quantitative reverse transcriptase PCR, representative agarose gel. iNOS expression was measured as the ratio of iNOS intensity versus GAPDH intensity.

View larger version (19K): [in a new window]   Figure 2 A, Relation between functional capacity (VO2max) and iNOS expression in skeletal muscle of patients with chronic heart failure (closed circles) and healthy normals (open circles). B, Relation between the relative amount of mi-CK and iNOS expression as determined by % positive tissue area in skeletal muscle of patients with chronic heart failure (closed circles) and healthy normals (open circles). Note the hyperbolic correlation.

View larger version (28K): [in a new window]   Figure 3 A, Representative Western blot showing the concentration-dependance of mi-CK expression with ascending doses of the NO-donator sodium nitroprusside (SNP). B, Dose-response relation between SNP and mi-CK expression in iNOS transfected L6 skeletal myoblasts. Mean of three experiments, mean ± SD.