This Article Abstract Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Armstrong, A. T. Articles by Leier, C. V. Search for Related Content PubMed PubMed Citation Articles by Armstrong, A. T. Articles by Leier, C. V.

Quantitative investigation of cardiomyocyte hypertrophy and myocardial fibrosis over 6 years after cardiac transplantation

Arthur T. Armstrong, PhD^*, Philip F. Binkley, MD, FACC^*, Peter B. Baker, MD, P. David Myerowitz, MD, FACC and Carl V. Leier, MD, FACC^*

^* Division of Cardiology, The Ohio State University College of Medicine, Columbus, Ohio, USA
Department of Pathology, The Ohio State University College of Medicine, Columbus, Ohio, USA
Division of Cardiac Surgery, The Ohio State University College of Medicine, Columbus, Ohio, USA

View larger version (93K): [in a new window]   Figure 1 A, Region of interest (sampling field) of an endomyocardial biopsy specimen (Masson trichrome stain). B, Perpendicular measurement lines were placed across sectioned horizontal cardiomyocytes at the level of midnucleus. The line lengths were measured and averaged by computer software to provide the mean myocyte diameter of the biopsy specimen. C, Using the operator’s threshold settings, the myocardial fibrous tissue was highlighted (blue) and measured by computer software as percent of myocardial tissue. The white nontissue spaces were similarly demarcated, measured and subtracted to provide a corrected area of true myocardial tissue.

View larger version (22K): [in a new window]   Figure 2 Mean (± 1 SD) cardiomyocyte diameters at serial time points after cardiac transplantation in 50 patients up to 60 months and 30 patients at 72 months. Data from 40 dilated cardiomyopathy (DCM) patients and 11 normal control subjects were obtained for comparison. *p < 0.05 vs. normal control; p < 0.05 vs. DCM; p < 0.05 vs. baseline (6 months after transplant).

View larger version (21K): [in a new window]   Figure 3 Mean (± 1 SD) percent fibrosis of endomyocardial biopsy specimens obtained serially after cardiac transplantation (n = 50 at 60 months and n = 30 at 72 months) and from 40 patients with primary dilated cardiomyopathy (DCM) and 11 normal control subjects. *p < 0.05 vs. normal control; p < 0.05 vs. DCM; p < 0.05 vs. baseline (1 month after transplant).