This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (1) Google Scholar Articles by Venge, P. Articles by James, S. PubMed Articles by Venge, P. Articles by James, S. Related Collections Related Articles

CLINICAL RESEARCH: BIOMARKERS

Normal Plasma Levels of Cardiac Troponin I Measured by the High-Sensitivity Cardiac Troponin I Access Prototype Assay and the Impact on the Diagnosis of Myocardial Ischemia

Per Venge, MD, PhD^_*,_*, Nina Johnston, MD, PhD, Bertil Lindahl, MD, PhD^, and Stefan James, MD, PhD^,

^_* Department of Medical Sciences, Clinical Chemistry, University of Uppsala, Uppsala, Sweden
Department of Cardiology, University of Uppsala, Uppsala, Sweden
Uppsala Clinical Research Centre, University of Uppsala, Uppsala, Sweden

Manuscript received February 5, 2009; revised manuscript received March 23, 2009, accepted May 4, 2009.

^_* Reprint requests and correspondence: Dr. Per Venge, Department of Clinical Chemistry and Pharmacology, University Hospital, SE-751 85 Uppsala, Sweden (Email: per.venge{at}medsci.uu.se).

Objectives: This study sought to evaluate the analytical and clinical performance of the novel hypersensitive cardiac troponin I (cTnI) prototype assay from Beckman Coulter (Fullerton, California).

Background: Studies on patients with acute coronary syndromes and on seemingly healthy subjects have shown that even very minor elevations of cardiac troponins are associated with an increased risk of death. However, the normal plasma levels of cardiac troponins are still not known.

Methods: cTnI plasma levels were measured in 542 healthy subjects, 319 men (age 59.9 ± 11.8 years) and 213 women (age 59.8 ± 13.1 years), and in 1,503 randomly selected patients of the GUSTO IV (Global Utilization of Strategies To open Occluded arteries IV) cohort with unstable angina and non–ST-segment elevation myocardial infarctions (MIs).

Results: The cTnI levels at 10% coefficient of variation and 20% coefficient of variation imprecision were 0.0033 and 0.0016 µg/l, respectively. The cTnI levels were measurable in >95% of the healthy subjects. The median level of healthy subjects <60 years of age was 0.0032 µg/l (range 0.0011 to 0.0079 µg/l) with the 99th percentile being 0.010 µg/l. No sex differences were observed. A receiver-operator characteristic curve analysis showed an optimal discrimination between healthy subjects and patients at 0.0064 µg/l with a sensitivity of 84.8% (95% confidence interval: 82.8% to 86.6%) and specificity of 89.7% (95% confidence interval: 86.8% to 92.2%). Outcomes as to death and/or MI were significantly different at this level (p < 0.01) in the GUSTO IV cohort.

Conclusions: The novel high-sensitivity cTnI prototype assay from Beckman Coulter allows for the first time the measurement of cTnI levels in almost all healthy subjects. Our data indicate that the assay may be a powerful aid in the diagnosis and outcome prediction of patients with suspected myocardial ischemia and question any definition of myocardial infarction.

Key Words: troponin I • unstable angina • myocardial infarction

Abbreviations and Acronyms   ACS = acute coronary syndrome   AMI = acute myocardial infarction   AUC = area under the curve   CI = confidence interval   cTnI = cardiac troponin I   cTnT = cardiac troponin T   CV = coefficient of variation   MI = myocardial infarction   ROC = receiver-operator characteristic   URL = upper reference limit

Related Articles

Highly Sensitive Troponins: The Answer or Just More Questions?

Judd E. Hollander
J. Am. Coll. Cardiol. 2009 54: 1173-1175. [Full Text] [PDF]

This article has been cited by other articles:

				J. E. Hollander Highly sensitive troponins the answer or just more questions? J. Am. Coll. Cardiol., September 22, 2009; 54(13): 1173 - 1175. [Full Text] [PDF]