CLINICAL RESEARCH: CARDIAC SURGERY
Plasma Tissue Factor Plus Activated Peripheral Mononuclear Cells Activate Factors VII and X in Cardiac Surgical Wounds
Takashi Hattori, MD*,
Mohammad M.H. Khan, MD, PhD ,
Robert W. Colman, MD and
L. Henry Edmunds, Jr, Md*,*
* Harrison Department of Surgical Research, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania
Sol Sherry Thrombosis Research Center, Hematology Division of the Departments of Medicine and Physiology, Temple University, Philadelphia, Pennsylvania
Manuscript received February 24, 2005;
revised manuscript received April 28, 2005,
accepted May 3, 2005.
* Reprint requests and correspondence: Dr. L. Henry Edmunds, Jr., 3440 Market Street, Philadelphia, Pennsylvania 19104-3325
(Email: hank.edmunds{at}uphs.upenn.edu).
OBJECTIVES: The purpose of this study was to test the hypothesis that activated monocytes with soluble plasma tissue factor (pTF) activate factors VII and X to generate thrombin.
BACKGROUND: Despite heparin, thrombin is progressively generated during cardiac surgery with cardiopulmonary bypass (CPB), produces intravascular fibrin and fibrinolysis, and causes serious thromboembolic and nonsurgical bleeding complications. Thrombin is primarily produced in the surgical wound, but mechanisms are unclear.
METHODS: In 13 patients, interactions of mononuclear cells, platelets, pTF, and pTF fractions to activate factors VII and X were evaluated in pre-bypass, perfusate, and pericardial wound blood before and during CPB.
RESULTS: Monocytes are activated in wound, but not in pre-bypass or perfusate plasma (monocyte chemotactic protein-1 = 29.5 ± 2.1 pmoles/l vs. 2.8 ± 1.2 pmoles/l and 3.3 ±1.4 pmoles/l, respectively). Wound pTF is substantially elevated compared to other locations (3.64 ± 0.45 pmoles/l vs. 0.71 ± 0.65 pmoles/l and 1.31 ± 1.4 pmoles/l). Supernatant wound pTF contains 81.7% of TF antigen; wound microparticle pTF contains 18.3%. Wound monocytes and all C5a-stimulated monocytes (but not activated platelets) completely convert factor VII to factor VIIa with wound pTF. Activated monocytes more efficiently activate factor X with wound supernatant TF/factor VII(VIIa) complex than with wound microparticle TF/factor VII(fVIIa). The correlation coefficient (r) between wound thrombin generation (F1.2) and wound pTF concentration is 0.944 (p = 0.0004).
CONCLUSIONS: During cardiac surgery with CPB, wound monocytes plus wound pTF or wound microparticle-free supernatant pTF preferentially accelerate activation of factor VII and factor X. This system represents a novel mechanism for thrombin generation via the TF coagulation pathway.
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Abbreviations and Acronyms
| | CPB = cardiopulmonary bypass | | ELISA = enzyme-linked immunosorbent assay | | fVII = factor VII | | fX = factor X | | IgG = immunoglobulin G | | MpTF = tissue factor on plasma microparticles | | PTF = soluble plasma tissue factor | | spTF = tissue factor in plasma supernatant | | TAT = thrombin antithrombin complex | | TF = tissue factor |
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