Prior exercise training improves the outcome of acute myocardial infarction in the rat
Heart structure, function, and gene expression
Sarit Freimann, PhD*, ,
Mickey Scheinowitz, PhD, ,
Daniel Yekutieli, PhD ,
Micha S. Feinberg, MD||,
Michael Eldar, MD|| and
Gania Kessler-Icekson, PhD*,*
* Felsenstein Medical Research Center, Tel-Aviv University, Tel-Aviv, Israel
Neufeld Cardiac Research Institute, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel
Department of Biomedical Engineering, Tel-Aviv University, Tel-Aviv, Israel
Department of Statistics and Operation Research, Tel-Aviv University, Tel-Aviv, Israel
|| The Heart Institute, Sheba Medical Center, Tel-Aviv University, Tel-Aviv, Israel
Manuscript received July 15, 2004;
revised manuscript received October 20, 2004,
accepted November 11, 2004.
* Reprint requests and correspondence: Dr. Gania Kessler-Icekson, Felsenstein Medical Research Center, Beilinson Campus, Rabin Medical Center, Petach Tikva 49100, Israel
(Email: icekson{at}post.tau.ac.il).
OBJECTIVES: The aim of this research was to investigate the structural, functional, and molecular features of the remodeling heart in prior swim-trained infarcted rats.
BACKGROUND: Physical exercise training is a known protective factor against cardiovascular morbidity and mortality. The structural and molecular aspects underlying this protection in the remodeling heart have not been investigated.
METHODS: After seven weeks of swimming exercise training, rats underwent surgical ligation of the left coronary artery followed by a four-week sedentary period. Untrained control rats underwent the same surgical protocol. Left ventricular function was assessed by echocardiography four weeks after infarction, and hearts were sampled for histological and molecular analysis. Ribonucleic acid from the surviving left ventricle was analyzed by complementary deoxyribonucleic acid arrays followed by Northern blotting or quantitative reverse transcription polymerase chain reaction of selected messenger ribonucleic acids (mRNAs).
RESULTS: Scar area was 1.6-fold smaller (p = 0.0002), arteriolar density was 1.7-fold higher (p = 0.0002), and left ventricular shortening fraction was 1.9-fold higher (p = 0.003) in the exercise-trained compared with sedentary hearts. Eleven genes whose expression level varied by at least ±1.5-fold distinguished the prior exercised rats from their sedentary counterparts. Compared with sedentary, the exercised hearts displayed 9- and 2.4-times lower levels of atrial natriuretic peptide and aldolase mRNA (p = 0.03 and 0.04, respectively), and a 2.7- and 1.9-fold higher abundance of cytochrome c-oxidase and fatty acid binding protein, respectively (p < 0.03, each).
CONCLUSIONS: Swimming exercise training before acute myocardial infarction reduces scar size, increases arteriole density, and manifests adaptation of stress- and energy-metabolism-related genes that may contribute to the improved heart function observed during remodeling.
|
Abbreviations and Acronyms
| | ANP = atrial natriuretic peptide | | COX = cytochrome c-oxidase | | Ex = exercise | | LV = left ventricle/ventricular | | LVEDD = left ventricular end-diastolic diameter | | LVESD = left ventricular end-systolic diameter | | MI = myocardial infarction | | mRNA = messenger ribonucleic acid | | qRT-PCR = quantitative reverse transcription polymerase chain reaction | | Sed = sedentary | | SF = shortening fraction |
|
Related Article
-
Benefit of an exercise program before myocardial infarction
- Robert A. Kloner and Boris Z. Simkhovich
J. Am. Coll. Cardiol. 2005 45: 939-940.
[Full Text]
[PDF]
This article has been cited by other articles:
|
|
|
|
 
M. C. de Waard and D. J. Duncker
Prior exercise improves survival, infarct healing, and left ventricular function after myocardial infarction
J Appl Physiol,
September 1, 2009;
107(3):
928 - 936.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Teerapornpuntakit, N. Dorkkam, K. Wongdee, N. Krishnamra, and N. Charoenphandhu
Endurance swimming stimulates transepithelial calcium transport and alters the expression of genes related to calcium absorption in the intestine of rats
Am J Physiol Endocrinol Metab,
April 1, 2009;
296(4):
E775 - E786.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Serra, M. L. Higuchi, S. S.M. Ihara, E. L. Antonio, M. H.H. Santos, M. T.N.M. Bombig, and P. J.F. Tucci
Exercise training prevents {beta}-adrenergic hyperactivity-induced myocardial hypertrophy and lesions
Eur J Heart Fail,
June 1, 2008;
10(6):
534 - 539.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Medeiros, N. P. L. Rolim, R. S. F. Oliveira, K. T. Rosa, K. C. Mattos, D. E. Casarini, M. C. Irigoyen, E. M. Krieger, J. E. Krieger, C. E. Negrao, et al.
Exercise training delays cardiac dysfunction and prevents calcium handling abnormalities in sympathetic hyperactivity-induced heart failure mice
J Appl Physiol,
January 1, 2008;
104(1):
103 - 109.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Brown and R. L. Moore
Perspectives in innate and acquired cardioprotection: cardioprotection acquired through exercise
J Appl Physiol,
November 1, 2007;
103(5):
1894 - 1899.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Ventura-Clapier, B. Mettauer, and X. Bigard
Beneficial effects of endurance training on cardiac and skeletal muscle energy metabolism in heart failure
Cardiovasc Res,
January 1, 2007;
73(1):
10 - 18.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. Domenech
Preconditioning: A New Concept About the Benefit of Exercise
Circulation,
January 3, 2006;
113(1):
e1 - e3.
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. D. Thompson
Exercise Prescription and Proscription for Patients With Coronary Artery Disease
Circulation,
October 11, 2005;
112(15):
2354 - 2363.
[Full Text]
[PDF]
|
|
|
|
