ACUTE CORONARY SYNDROMES
Proteomic analysis of plasma from patients during an acute coronary syndrome
Petra J. Mateos-Cáceres, PhD*,
Antonio García-Méndez, PhD*,
Antonio López Farré, PhD*,
Carlos Macaya, MD, PhD*,
Antonio Núñez, MD, PhD,*,
Juan Gómez, MD, PhD*,
Sergio Alonso-Orgaz, PhD*,
Carolina Carrasco, PhD*,
Marta Escribano Burgos, PhD*,
Raimundo de Andrés, MD ,
Juan J. Granizo, MD ,
Jerónimo Farré, MD,* and
Luis A. Rico, MD, PhD*,*
* Cardiovascular Institute, Hospital Clínico San Carlos, Madrid, Spain
Emergency Unit
Epidemiology Department, Cardiovascular Research Laboratory, Fundación Jiménez Díaz, Madrid, Spain
Manuscript received December 16, 2003;
revised manuscript received April 21, 2004,
accepted June 16, 2004.
* Reprint requests and correspondence: Dr. Luis A. Rico, Cardiovascular Research Laboratory, Fundación Jiménez Díaz, Avda. Reyes Catolicos, 2, Madrid 28040, Spain
(Email: rico{at}fjd.es).
OBJECTIVES: The aim of this study was to analyze modifications in the plasma protein map during an acute coronary syndrome (ACS) using proteomics.
BACKGROUND: Proteomics is a new technology that allows the detection and identification of several proteins at a given time in a sample.
METHODS: Plasma from 19 patients, 11 with acute myocardial infarction (AMI) and 8 with unstable angina (UA), was investigated. The control group included nine age-matched volunteers.
RESULTS: In two-dimensional electrophoresis using a pH range of 4 to 7, constant differences were found in at least four different areas within the plasma protein map. In area 1, we identified the presence of seven alpha1-antitrypsin (AAT) isoforms in plasma from control subjects. alpha1-antitrypsin isoform 1 was undetectable in plasma from UA and AMI patients. The AAT isoforms 5, 6, and 7 were reduced in plasma from AMI patients when compared with UA patients. Three fibrinogen gamma chain isoforms were identified in area 2. Fibrinogen gamma chain isoforms 1 and 2 were increased in AMI patients with respect to UA patients. Five apolipoprotein A-I isoforms were identified in area 3. All of them were reduced in plasma from AMI patients with respect to UA patients. In area 4, the  -immunoglobulin heavy chains were detected and were found increased in plasma from ACS patients.
CONCLUSIONS: Plasma proteomic analysis makes it possible to develop a map of the protein isoforms that are expressed in plasma during an ACS.
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Abbreviations and Acronyms
| | AAT = alpha1-antitrypsin | | ACS = acute coronary syndrome | | AMI = acute myocardial infarction | | Apo A-I = apolipoprotein A-I | | 2-DE = bidimensional electrophoresis | | MALDI-TOF MS = matrix-assisted laser desorption/ionization-time of flight mass spectrometry | | MS/MS = tandem mass spectrometry | | UA = unstable angina |
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