HOME SUBSCRIPTIONS CURRENT ISSUE PAST ISSUES CARDIOSOURCE SEARCH HELP FEEDBACK		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Christman, K. L. Articles by Lee, R. J. Search for Related Content PubMed PubMed Citation Articles by Christman, K. L. Articles by Lee, R. J.

Injectable fibrin scaffold improves cell transplant survival, reduces infarct expansion, and induces neovasculature formation in ischemic myocardium

Karen L. Christman, BS^*, Andrew J. Vardanian, MS, Qizhi Fang, MD, Richard E. Sievers, BS, Hubert H. Fok, MD and Randall J. Lee, MD, PhD^*,*

^* University of California Berkeley and San Francisco Joint Bioengineering Graduate Group, University of California San Francisco, San Francisco, California, USA
Department of Medicine, University of California San Francisco, San Francisco, California, USA
Cardiovascular Research Institute, University of California San Francisco, San Francisco, California, USA

Manuscript received December 2, 2003; revised manuscript received March 22, 2004, accepted April 13, 2004.

^* Reprint requests and correspondence: Dr. Randall J. Lee, Cardiac Electrophysiology, MU East Tower, Box 1354, 500 Parnassus Avenue, San Francisco, California 94143-1354, USA.
lee{at}medicine.ucsf.edu

OBJECTIVES: In this study, we determined whether fibrin glue improves cell transplant retention and survival, reduces infarct expansion, and induces neovasculature formation.

BACKGROUND: Current efforts in restoring the myocardium after myocardial infarction (MI) include the delivery of viable cells to replace necrotic cardiomyocytes. Cellular transplantation techniques are, however, limited by transplanted cell retention and survival within the ischemic tissue.

METHODS: The left coronary artery of rats was occluded for 17 min followed by reperfusion. One week later, bovine serum albumin (BSA), fibrin glue, skeletal myoblasts in BSA, or skeletal myoblasts in fibrin glue were injected into the infarcted area of the left ventricle. The animals were euthanized five weeks after injection, and their hearts were excised, fresh frozen, and sectioned for histology and immunohistochemistry.

RESULTS: After five weeks, the mean area covered by skeletal myoblasts in fibrin glue was significantly greater than the area covered by myoblasts injected in BSA. Myoblasts within the infarct were often concentrated around arterioles. The infarct scar size and myoblasts in the fibrin group were significantly smaller than those in the control and BSA groups. Fibrin glue also significantly increased the arteriole density in the infarct scar as compared with the control group.

CONCLUSIONS: This study indicates that fibrin glue increases cell transplant survival, decreases infarct size, and increases blood flow to ischemic myocardium. Therefore, fibrin glue may have potential as a biomaterial scaffold to improve cellular cardiomyoplasty treat and MIs.

Abbreviations and Acronyms   BSA = bovine serum albumin   DAPI = 4',6-diamidino-2-phenylindole   LV = left ventricle   MHC = myosin heavy chain   MI = myocardial infarction   PBS = phosphate-buffered saline

This article has been cited by other articles:

				H. Jawad, A. R. Lyon, S. E. Harding, N. N. Ali, and A. R. Boccaccini Myocardial tissue engineering Br. Med. Bull., September 1, 2008; 87(1): 31 - 47. [Abstract] [Full Text] [PDF]

				P. Akhyari, H. Kamiya, A. Haverich, M. Karck, and A. Lichtenberg Myocardial tissue engineering: the extracellular matrix. Eur. J. Cardiothorac. Surg., August 1, 2008; 34(2): 229 - 241. [Abstract] [Full Text] [PDF]

				N. Landa, L. Miller, M. S. Feinberg, R. Holbova, M. Shachar, I. Freeman, S. Cohen, and J. Leor Effect of Injectable Alginate Implant on Cardiac Remodeling and Function After Recent and Old Infarcts in Rat Circulation, March 18, 2008; 117(11): 1388 - 1396. [Abstract] [Full Text] [PDF]

				R. J. Lee, Q. Fang, P. A. Davol, Y. Gu, R. E. Sievers, R. C. Grabert, J. M. Gall, E. Tsang, M. S. Yee, H. Fok, et al. Antibody Targeting of Stem Cells to Infarcted Myocardium Stem Cells, March 1, 2007; 25(3): 712 - 717. [Abstract] [Full Text] [PDF]

				K. L. Christman and R. J. Lee Biomaterials for the Treatment of Myocardial Infarction J. Am. Coll. Cardiol., September 5, 2006; 48(5): 907 - 913. [Abstract] [Full Text] [PDF]

				E. J. Suuronen, J. P. Veinot, S. Wong, V. Kapila, J. Price, M. Griffith, T. G. Mesana, and M. Ruel Tissue-Engineered Injectable Collagen-Based Matrices for Improved Cell Delivery and Vascularization of Ischemic Tissue Using CD133+ Progenitors Expanded From the Peripheral Blood Circulation, July 4, 2006; 114(1_suppl): I-138 - I-144. [Abstract] [Full Text] [PDF]

				V. Chekanov, N. Kipshidze, and V. Nikolaychik Cell Transplantation and Fibrin Matrix J. Am. Coll. Cardiol., May 17, 2005; 45(10): 1734 - 1735. [Full Text] [PDF]

				K. L. Christman and R. J. Lee Reply J. Am. Coll. Cardiol., May 17, 2005; 45(10): 1735 - 1736. [Full Text] [PDF]