This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Palmerini, T. Articles by Coller, B. S. Search for Related Content PubMed PubMed Citation Articles by Palmerini, T. Articles by Coller, B. S.

EXPERIMENTAL STUDY

Effects of abciximab on the acute pathology of blood vessels after arterial stenting in nonhuman primates

Tullio Palmerini, MD^*, Mark A. Nedelman, MS, Lesley E. Scudder, BSc, Marian T. Nakada, PhD^||, Robert E. Jordan, PhD^||, Susan Smyth, MD, PhD, Ronald E. Gordon, PhD, John T. Fallon, MD, PhD and Barry S. Coller, MD^,*

^* Istituto di Cardiologia, Policlinico S. Orsola, University of Bologna, Bologna, Italy
Primedica Corporation, Worcester, Massachusetts, USA
Medicine, New York, New York, USA
Pathology, Mount Sinai Medical School, New York, New York, USA
^|| Centocor, Malvern, Pennsylvania, USA

Manuscript received September 18, 2001; revised manuscript received April 8, 2002, accepted April 18, 2002.

^* Reprint requests and correspondence: Dr. Barry S. Coller, Head, Laboratory of Blood and Vascular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.
collerb{at}rockefeller.edu

OBJECTIVES: This study was designed to assess the effect of abciximab on platelet and leukocyte deposition 60 min after stent insertion in nonhuman primates.

BACKGROUND: Although it is well established that abciximab improves both short- and long-term clinical outcomes after stent placement, there have been no studies assessing its effect on early platelet and leukocyte deposition.

METHODS: Cynomolgus monkeys were pretreated with aspirin and either saline or a 0.4 mg/kg bolus of abciximab, and then subjected to angioplasty and Palmaz-Schatz stent placement in the common iliac artery or abdominal aorta. After 60 min, animals were euthanized and the stented artery was evaluated by immunohistochemistry and morphometry.

RESULTS: Complete occlusion of the stented vessel with a thin fibrin(ogen) meshwork and trapped blood occurred in two saline-treated and two abciximab-treated animals. In the four remaining saline-treated animals, a layer of erythrocytes trapped in a network of fibrin(ogen) was noted close to the vessel wall, and this was covered by a layer of large, irregular platelet thrombi. Leukocytes formed a monolayer on top of the platelets and near stent struts. In the four remaining abciximab-treated animals, the mean erythrocyte area was 65% smaller (p = 0.070), the platelet aggregate area was 89% smaller (p = 0.049) and the luminal area was 59% larger (p = 0.004). A monolayer of leukocytes also formed on top of the platelets and near stent struts.

CONCLUSIONS: In control stented blood vessels in this study, platelet thrombi formed not at the vessel wall, but on top of an erythrocyte-rich layer, and platelets recruited leukocytes. Abciximab decreased the size of platelet thrombi, but did not prevent leukocyte recruitment.

Abbreviations and Acronyms   GP   glycoprotein   IEL   internal elastic lamina   PAI-1   plasminogen activating inhibitor-1   PBS   phosphate-buffered saline   PSGL-1   P-selectin glycoprotein ligand-1   RPFA   rapid platelet function assay   TF   tissue factor   t-PA   tissue plasminogen activator

This article has been cited by other articles:

				G. A. Zimmerman and A. S. Weyrich Signal-Dependent Protein Synthesis by Activated Platelets: New Pathways to Altered Phenotype and Function Arterioscler Thromb Vasc Biol, March 1, 2008; 28(3): s17 - s24. [Abstract] [Full Text] [PDF]

				A. S. Weyrich, M. M. Denis, H. Schwertz, N. D. Tolley, J. Foulks, E. Spencer, L. W. Kraiss, K. H. Albertine, T. M. McIntyre, and G. A. Zimmerman mTOR-dependent synthesis of Bcl-3 controls the retraction of fibrin clots by activated human platelets Blood, March 1, 2007; 109(5): 1975 - 1983. [Abstract] [Full Text] [PDF]

				T. Palmerini, A. Marzocchi, C. Marrozzini, P. Ortolani, F. Saia, L. Bacchi-Reggiani, S. Virzi, S. Gianstefani, and A. Branzi Preprocedural Levels of C-Reactive Protein and Leukocyte Counts Predict 9-Month Mortality After Coronary Angioplasty for the Treatment of Unprotected Left Main Coronary Artery Stenosis Circulation, October 11, 2005; 112(15): 2332 - 2338. [Abstract] [Full Text] [PDF]

				T. Palmerini, B. S. Coller, V. Cervi, L. Tomasi, A. Marzocchi, C. Marrozzini, O. Leone, M. Piccioli, and A. Branzi Monocyte-derived tissue factor contributes to stent thrombosis in an in vitro system J. Am. Coll. Cardiol., October 19, 2004; 44(8): 1570 - 1577. [Abstract] [Full Text] [PDF]