CLINICAL STUDY: ATHEROSCLEROSIS
Postprandial endothelial activation in healthy subjects and in type 2 diabetic patients: Role of fat and carbohydrate meals
Francesco Nappo, MD*,
Katherine Esposito, MD ,
Michele Cioffi, MD,
Giovanni Giugliano*,
Anna Maria Molinari, MD,
Giuseppe Paolisso, MD*,
Raffaele Marfella, MD* and
Dario Giugliano, MD*,*
* Department of Geriatrics and Metabolic Diseases, Second University of Naples, Naples, Italy
Cardiovascular Research Center, Second University of Naples, Naples, Italy
Department of General Pathology, Chair of Clinical Pathology, Second University of Naples, Naples, Italy
Manuscript received August 10, 2001;
revised manuscript received December 18, 2001,
accepted January 9, 2002.
* Reprint requests and correspondence: Dr. Dario Giugliano, Via Emilia 1, 80021 AFRAGOLA (NA), Italy.
dario.giugliano{at}unina2.it
OBJECTIVES: To compare the effect of a high-fat meal and a high-carbohydrate meal (pizza), with and without antioxidant vitamins, on endothelial activation in healthy subjects and in patients with type 2 diabetes mellitus.
BACKGROUND: The postprandial state is becoming increasingly acknowledged to affect some early events of atherogenesis.
METHODS: In a randomized, observer-blinded, crossover study, 20 newly diagnosed type 2 diabetic patients and 20 age- and gender-matched healthy subjects received two meals at one-week intervals: a high-fat meal (760 calories) and an isoenergetic high-carbohydrate meal (non-cheese pizza). In all subjects, the same meals were repeated immediately following ingestion of vitamin E, 800 IU, and ascorbic acid, 1,000 mg.
RESULTS: In normal subjects, the high-fat meal increased the plasma levels of tumor necrosis factor- (TNF-), interleukin-6 (IL-6), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), which were prevented by vitamins. No change in these parameters occurred after pizza ingestion or pizza ingestion with vitamins. In diabetic patients, basal concentrations of glucose, cytokines and adhesion molecules were significantly higher than in nondiabetic controls. Both meals significantly increased cytokine and adhesion molecule levels, but the increase was more sustained following the high-fat meal. There was no significant change from baseline when vitamin supplementation accompanied each meal. There was a relationship between changes in serum triglycerides and changes in TNF- (r = 0.39, p < 0.01), IL-6 (r = 0.28, p < 0.05) and VCAM-1 (r = 0.25, p < 0.05), and between changes in plasma glucose and changes in IL-6 (r = 0.36, p < 0.01) and ICAM-1 (r = 0.31, p < 0.02).
CONCLUSIONS: An oxidative mechanism mediates endothelial activation induced by post-meal hyperlipidemia and hyperglycemia.
|
Abbreviations and Acronyms
| | ICAM-1 | | intercellular adhesion molecule-1 | | IL-6 | | interleukin-6 | | TNF- | | tumor necrosis factor-alpha | | VCAM-1 | | vascular cell adhesion molecule-1 |
|
This article has been cited by other articles:
|
|
|
|
 
D. Thompson and N. Dixon
Measurement of postprandial interleukin-6 by using a catheter: what does it tell us?
Am. J. Clinical Nutrition,
November 1, 2009;
90(5):
1446 - 1446.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Davidson and C. G. Parkin
Is Hyperglycemia a Causal Factor in Cardiovascular Disease?: Does proving this relationship really matter? Yes
Diabetes Care,
November 1, 2009;
32(suppl_2):
S331 - S333.
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. C. Dixon, T. L. Hurst, D. C. S. Talbot, R. M. Tyrrell, and D. Thompson
Active middle-aged men have lower fasting inflammatory markers but the postprandial inflammatory response is minimal and unaffected by physical activity status
J Appl Physiol,
July 1, 2009;
107(1):
63 - 68.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A Alvarez, P. B Higgins, R. A Oster, J. R Fernandez, B. E Darnell, and B. A Gower
Fasting and postprandial markers of inflammation in lean and overweight children
Am. J. Clinical Nutrition,
April 1, 2009;
89(4):
1138 - 1144.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. E. Berry, S. Tucker, R. Banerji, B. Jiang, P. J. Chowienczyk, S. M. Charles, and T. A. B. Sanders
Impaired Postprandial Endothelial Function Depends on the Type of Fat Consumed by Healthy Men
J. Nutr.,
October 1, 2008;
138(10):
1910 - 1914.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. L. Hall, K. A. Sanders, T. A. B. Sanders, and P. J. Chowienczyk
A High-Fat Meal Enriched with Eicosapentaenoic Acid Reduces Postprandial Arterial Stiffness Measured by Digital Volume Pulse Analysis in Healthy Men
J. Nutr.,
February 1, 2008;
138(2):
287 - 291.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Peter and A. Rees
Review: Postprandial glycaemia and cardiovascular risk
The British Journal of Diabetes & Vascular Disease,
January 1, 2008;
8(1):
8 - 14.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. Plat, A. Jellema, J. Ramakers, and R. P. Mensink
Weight Loss, but Not Fish Oil Consumption, Improves Fasting and Postprandial Serum Lipids, Markers of Endothelial Function, and Inflammatory Signatures in Moderately Obese Men
J. Nutr.,
December 1, 2007;
137(12):
2635 - 2640.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Erridge, T. Attina, C. M Spickett, and D. J Webb
A high-fat meal induces low-grade endotoxemia: evidence of a novel mechanism of postprandial inflammation
Am. J. Clinical Nutrition,
November 1, 2007;
86(5):
1286 - 1292.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Mita, H. Watada, T. Shimizu, Y. Tamura, F. Sato, T. Watanabe, J. B. Choi, T. Hirose, Y. Tanaka, and R. Kawamori
Nateglinide Reduces Carotid Intima-Media Thickening in Type 2 Diabetic Patients Under Good Glycemic Control
Arterioscler Thromb Vasc Biol,
November 1, 2007;
27(11):
2456 - 2462.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. H St-Pierre, J.-P. Bastard, L. Coderre, M. Brochu, A. D Karelis, M.-E. Lavoie, F. Malita, J. Fontaine, D. Mignault, K. Cianflone, et al.
Association of acylated ghrelin profiles with chronic inflammatory markers in overweight and obese postmenopausal women: a MONET study
Eur. J. Endocrinol.,
October 1, 2007;
157(4):
419 - 426.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. H. McNulty, M. A. Tulli, B. J. Robertson, V. Lendel, L. A. Harach, S. Scott, and J. P. Boehmer
Effect of simulated postprandial hyperglycemia on coronary blood flow in cardiac transplant recipients
Am J Physiol Heart Circ Physiol,
July 1, 2007;
293(1):
H103 - H108.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J Hamilton, G. T Chew, and G. F Watts
Therapeutic regulation of endothelial dysfunction in type 2 diabetes mellitus
Diabetes and Vascular Disease Research,
June 1, 2007;
4(2):
89 - 102.
[Abstract]
[PDF]
|
|
|
|
|
|
|
F. Mariotti, J. F. Huneau, I. Szezepanski, K. J. Petzke, Y. Aggoun, D. Tome, and D. Bonnet
Meal Amino Acids with Varied Levels of Arginine do Not Affect Postprandial Vascular Endothelial Function in Healthy Young Men
J. Nutr.,
June 1, 2007;
137(6):
1383 - 1389.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Tushuizen, R. Nieuwland, C. Rustemeijer, B. E. Hensgens, A. Sturk, R. J. Heine, and M. Diamant
Elevated Endothelial Microparticles Following Consecutive Meals Are Associated With Vascular Endothelial Dysfunction in Type 2 Diabetes
Diabetes Care,
March 1, 2007;
30(3):
728 - 730.
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. J. Ignarro, M. L. Balestrieri, and C. Napoli
Nutrition, physical activity, and cardiovascular disease: An update
Cardiovasc Res,
January 15, 2007;
73(2):
326 - 340.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Giugliano, A. Ceriello, and K. Esposito
The Effects of Diet on Inflammation: Emphasis on the Metabolic Syndrome
J. Am. Coll. Cardiol.,
August 15, 2006;
48(4):
677 - 685.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Giugliano and K. Esposito
Virgin Olive Oil and Vegetables Improve Endothelial Health
J. Am. Coll. Cardiol.,
July 18, 2006;
48(2):
413 - 414.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Maggio, J. M. Guralnik, D. L. Longo, and L. Ferrucci
Interleukin-6 in aging and chronic disease: a magnificent pathway.
J. Gerontol. A Biol. Sci. Med. Sci.,
June 1, 2006;
61(6):
575 - 584.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Basu, S. Devaraj, and I. Jialal
Dietary Factors That Promote or Retard Inflammation
Arterioscler Thromb Vasc Biol,
May 1, 2006;
26(5):
995 - 1001.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Shimabukuro, N. Higa, I. Chinen, K. Yamakawa, and N. Takasu
Effects of a Single Administration of Acarbose on Postprandial Glucose Excursion and Endothelial Dysfunction in Type 2 Diabetic Patients: A Randomized Crossover Study
J. Clin. Endocrinol. Metab.,
March 1, 2006;
91(3):
837 - 842.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Esposito and D. Giugliano
Diet and inflammation: a link to metabolic and cardiovascular diseases
Eur. Heart J.,
January 1, 2006;
27(1):
15 - 20.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Ahmed, R. Babaei-Jadidi, S. K. Howell, P. J. Thornalley, and P. J. Beisswenger
Glycated and Oxidized Protein Degradation Products Are Indicators of Fasting and Postprandial Hyperglycemia in Diabetes
Diabetes Care,
October 1, 2005;
28(10):
2465 - 2471.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Corpeleijn, W. H. M. Saris, E. H. J. M. Jansen, P. M. H. J. Roekaerts, E. J. M. Feskens, and E. E. Blaak
Postprandial Interleukin-6 Release from Skeletal Muscle in Men with Impaired Glucose Tolerance Can Be Reduced by Weight Loss
J. Clin. Endocrinol. Metab.,
October 1, 2005;
90(10):
5819 - 5824.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Giugliano, R. Marfella, K. Esposito, A. C. Ferreira, A. A. Peter, J. A. Chirinos, R. Ghany, S. Virani, S. Garcia, J. Purow, et al.
Letter Regarding Article by Ferriera et al, "Postprandial Hypertriglyceridemia Increases Circulating Levels of Endothelial Cell Microparticles" * Response
Circulation,
June 28, 2005;
111(25):
e457 - e458.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Ceriello, R. Assaloni, R. Da Ros, A. Maier, L. Piconi, L. Quagliaro, K. Esposito, and D. Giugliano
Effect of Atorvastatin and Irbesartan, Alone and in Combination, on Postprandial Endothelial Dysfunction, Oxidative Stress, and Inflammation in Type 2 Diabetic Patients
Circulation,
May 17, 2005;
111(19):
2518 - 2524.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. A. Tataranni and E. Ortega
A Burning Question: Does an Adipokine-Induced Activation of the Immune System Mediate the Effect of Overnutrition on Type 2 Diabetes?
Diabetes,
April 1, 2005;
54(4):
917 - 927.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. R. Skilton, N. T. Lai, K. A. Griffiths, L. M. Molyneaux, D. K. Yue, D. R. Sullivan, and D. S. Celermajer
Meal-related increases in vascular reactivity are impaired in older and diabetic adults: insights into roles of aging and insulin in vascular flow
Am J Physiol Heart Circ Physiol,
March 1, 2005;
288(3):
H1404 - H1410.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Ceriello
Postprandial Hyperglycemia and Diabetes Complications: Is It Time to Treat?
Diabetes,
January 1, 2005;
54(1):
1 - 7.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. A. Economides, L. Khaodhiar, A. Caselli, A. E. Caballero, H. Keenan, S.-E. Bursell, G. L. King, M. T. Johnstone, E. S. Horton, and A. Veves
The Effect of Vitamin E on Endothelial Function of Micro- and Macrocirculation and Left Ventricular Function in Type 1 and Type 2 Diabetic Patients
Diabetes,
January 1, 2005;
54(1):
204 - 211.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M E Tushuizen, M Diamant, and R J Heine
Postprandial dysmetabolism and cardiovascular disease in type 2 diabetes
Postgrad. Med. J.,
January 1, 2005;
81(951):
1 - 6.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M.R. Gill, A. Al-Mamari, W. R. Ferrell, S. J. Cleland, C. J. Packard, N. Sattar, J. R. Petrie, and M. J. Caslake
Effects of prior moderate exercise on postprandial metabolism and vascular function in lean and centrally obese men
J. Am. Coll. Cardiol.,
December 21, 2004;
44(12):
2375 - 2382.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Manning, W. H. Sutherland, G. Hendry, S. A. de Jong, M. McGrath, and S. M. Williams
Changes in Circulating Postprandial Proinflammatory Cytokine Concentrations in Diet-Controlled Type 2 Diabetes and the Effect of Ingested Fat
Diabetes Care,
October 1, 2004;
27(10):
2509 - 2511.
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Esposito, R. Marfella, M. Ciotola, C. Di Palo, F. Giugliano, G. Giugliano, M. D'Armiento, F. D'Andrea, and D. Giugliano
Effect of a Mediterranean-Style Diet on Endothelial Dysfunction and Markers of Vascular Inflammation in the Metabolic Syndrome: A Randomized Trial
JAMA,
September 22, 2004;
292(12):
1440 - 1446.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. P. Zhao, L. Liu, Y. C. Cheng, M. H. Shishehbor, M. H. Liu, D. Q. Peng, and Y. L. Li
Xuezhikang, an Extract of Cholestin, Protects Endothelial Function Through Antiinflammatory and Lipid-Lowering Mechanisms in Patients With Coronary Heart Disease
Circulation,
August 24, 2004;
110(8):
915 - 920.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Esposito, D. Giugliano, F. Nappo, R. Marfella, and for the Campanian Postprandial Hyperglycemia Study
Regression of Carotid Atherosclerosis by Control of Postprandial Hyperglycemia in Type 2 Diabetes Mellitus
Circulation,
July 13, 2004;
110(2):
214 - 219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Ceriello and E. Motz
Is Oxidative Stress the Pathogenic Mechanism Underlying Insulin Resistance, Diabetes, and Cardiovascular Disease? The Common Soil Hypothesis Revisited
Arterioscler Thromb Vasc Biol,
May 1, 2004;
24(5):
816 - 823.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. Ceriello, L. Quagliaro, L. Piconi, R. Assaloni, R. Da Ros, A. Maier, K. Esposito, and D. Giugliano
Effect of Postprandial Hypertriglyceridemia and Hyperglycemia on Circulating Adhesion Molecules and Oxidative Stress Generation and the Possible Role of Simvastatin Treatment
Diabetes,
March 1, 2004;
53(3):
701 - 710.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Ohshita, K. Yamane, M. Hanafusa, H. Mori, K. Mito, M. Okubo, H. Hara, and N. Kohno
Elevated White Blood Cell Count in Subjects With Impaired Glucose Tolerance
Diabetes Care,
February 1, 2004;
27(2):
491 - 496.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Esposito, F. Nappo, F. Giugliano, C. Di Palo, M. Ciotola, M. Barbieri, G. Paolisso, and D. Giugliano
Meal modulation of circulating interleukin 18 and adiponectin concentrations in healthy subjects and in patients with type 2 diabetes mellitus
Am. J. Clinical Nutrition,
December 1, 2003;
78(6):
1135 - 1140.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Ros
Dietary cis-monounsaturated fatty acids and metabolic control in type 2 diabetes
Am. J. Clinical Nutrition,
September 1, 2003;
78(3):
617S - 625.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. R. Gill, M. J. Caslake, C. McAllister, F. Tsofliou, W. R. Ferrell, C. J. Packard, and D. Malkova
Effects of Short-Term Detraining on Postprandial Metabolism, Endothelial Function, and Inflammation in Endurance-Trained Men: Dissociation between Changes in Triglyceride Metabolism and Endothelial Function
J. Clin. Endocrinol. Metab.,
September 1, 2003;
88(9):
4328 - 4335.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Liu, S.-P. Zhao, Y.-C. Cheng, and Y.-L. Li
Xuezhikang Decreases Serum Lipoprotein(a) and C-reactive Protein Concentrations in Patients with Coronary Heart Disease
Clin. Chem.,
August 1, 2003;
49(8):
1347 - 1352.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. F. Carroll and D. S. Schade
Timing of Antioxidant Vitamin Ingestion Alters Postprandial Proatherogenic Serum Markers
Circulation,
July 8, 2003;
108(1):
24 - 31.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Esposito, R. Marfella, and D. Giugliano
Stress Hyperglycemia, Inflammation, and Cardiovascular Events
Diabetes Care,
May 1, 2003;
26(5):
1650 - 1651.
[Full Text]
[PDF]
|
|
|
|
|
|
|
W.-Y. Chung, D.-W. Sohn, Y.-J. Kim, S. Oh, I. n-H. o Chai, Y.-B. Park, and Y.-S. Choi
Absence of postprandial surge in coronary blood flow distal to significant stenosis: a possible mechanism of postprandial angina
J. Am. Coll. Cardiol.,
December 4, 2002;
40(11):
1976 - 1983.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
