CLINICAL STUDY: PHARMACOLOGY
Differential effects of pentaerythritol tetranitrate and nitroglycerin on the development of tolerance and evidence of lipid peroxidation: a human in vivo study
Ursula Jurt, MD*  ,
Tommaso Gori, MD*  ,
Amir Ravandi, PhD ,
Saeid Babaei, MSc,
Peter Zeman* and
John D. Parker, MD, FACC*
* Division of Cardiology, Department of Medicine, Mount Sinai Hospital, Toronto, Canada
University Health Network, Toronto, Canada
Cardiovascular Collaborative Program, Toronto, Canada
Department of Laboratory Medicine and Pathobiology, Banting and Best Department of Medical Research, University of Toronto, Toronto, Canada
Manuscript received October 17, 2000;
revised manuscript received April 23, 2001,
accepted May 15, 2001.
Reprint requests and correspondence: Dr. John D. Parker, Division of Cardiology, Department of Medicine, Mount Sinai Hospital, Suite 1609, 600 University Avenue, Toronto, Ontario, Canada, M5G 1X5
jdp{at}inforamp.net
OBJECTIVES
We investigated the development of nitrate tolerance after continuous exposure to nitroglycerin (GTN) as compared with pentaerythritol tetranitrate (PETN) in humans.
BACKGROUND
Sustained therapy with GTN causes tolerance and has been associated with increased production of free oxygen radicals by the endothelium. Pentaerythritol tetranitrate is an organic nitrate that has been used in the therapy of angina. There have been no investigations concerning the development of tolerance to PETN in humans. Animal investigations suggested that continuous therapy with PETN does not cause increased free radical production or hemodynamic tolerance.
METHODS
We randomized 30 healthy volunteers to continuous GTN (0.6 mg/h/24 h), long-acting PETN (60 mg orally three times a day) or no treatment (control group) for seven days. We studied systemic blood pressure responses and venous volume responses to GTN with strain-gauge plethysmography. The levels of cytotoxic aldehydes and isoprostanes were measured as markers of free radical-mediated lipid peroxidation.
RESULTS
Tolerance, as demonstrated by blood pressure and forearm plethysmography, developed in the GTN group and was absent in the PETN group (p < 0.05). Therapy with GTN was associated with a significant increase in plasma markers of lipid peroxidation. This response was not observed in those treated with PETN (isoprostanes: control: 38 ± 5; GTN: 59 ± 6; PETN: 38 ± 3 µg/ml; p < 0.005).
CONCLUSIONS
Treatment with PETN does not cause tolerance and is not associated with evidence of increased free radical production.
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Abbreviations and Acronyms
| | ANOVA | = analysis of variance | | ES/MS | = on-line electrospray mass spectrometry | | FBV | = forearm venous blood volume | | GTN | = nitroglycerin | | HPLC | = high-pressure liquid chromatography | | NO | = nitric oxide | | PETN | = pentaerythritol tetranitrate | | ROS | = reactive oxygen species |
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