EXPERIMENTAL STUDY
Transendocardial delivery of autologous bone marrow enhances collateral perfusion and regional function in pigs with chronic experimental myocardial ischemia
Shmuel Fuchs, MD*,
Richard Baffour, PhD*,
Yi Fu Zhou, MD*,
Matie Shou, MD*,
Anthony Pierre, BSc*,
Fermin O. Tio, MD ,
Neil J. Weissman, MD*,
Martin B. Leon, MD ,
Stephen E. Epstein, MD* and
Ran Kornowski, MD*
* the Cardiovascular Research Institute, Washington, DC, USA
Biomedical Research Foundation of South Texas Inc., San-Antonio, Texas, USA
Cardiovascular Research Foundation, Lenox Hill Hospital, New York, New York, USA
Manuscript received December 12, 2000;
revised manuscript received January 9, 2001,
accepted January 24, 2001.
Reprint requests and correspondence: Dr. Shmuel Fuchs, Cardiovascular Research Institute, Washington Hospital Center, 110 Irving St. Northwest, 4B-1, Washington, DC 20010
sxf6{at}mhg.edu
OBJECTIVES
We tested the hypothesis that intramyocardial injection of autologous bone marrow (ABM) promotes collateral development in ischemic porcine myocardium. We also defined, in vitro, whether bone marrow (BM) cells secrete vascular endothelial growth factor (VEGF) and macrophage chemoattractant protein-1 (MCP-1).
BACKGROUND
The natural processes leading to collateral development are extremely complex, requiring multiple growth factors interacting in concert and in sequence. Because optimal angiogenesis may, therefore, require multiple angiogenic factors, we thought that injection of BM, which contains cells that secrete numerous angiogenic factors, might provide optimal therapeutic angiogenesis.
METHODS
Bone marrow was cultured four weeks in vitro. Conditioned medium was assayed for VEGF and MCP-1 and was added to cultured pig aortic endothelial cells (PAEC) to assess proliferation. Four weeks after left circumflex ameroid implantation, freshly aspirated ABM (n = 7) or heparinized saline (n = 7) was injected transendocardially into the ischemic zone (0.2 ml/injection at 12 sites). Echocardiography to assess myocardial thickening and microspheres to assess perfusion were performed at rest and during stress.
RESULTS
Vascular endothelial growth factor and MCP-1 concentrations increased in a time-related manner. The conditioned medium enhanced, in a dose-related manner, PAEC proliferation. Collateral flow (ischemic/normal zone x 100) improved in ABM-treated pigs (ABM: 98 ± 14 vs. 83 ± 12 at rest, p = 0.001; 89 ± 18 vs. 78 ± 12 during adenosine, p = 0.025; controls: 92 ± 10 vs. 89 ± 9 at rest, p = 0.49; 78 ± 11 vs. 77 ± 5 during adenosine, p = 0.75). Similarly, contractility increased in ABM-treated pigs (ABM: 83 ± 21 vs. 60 ± 32 at rest, p = 0.04; 91 ± 44 vs. 36 ± 43 during pacing, p = 0.056; controls: 69 ± 48 vs. 64 ± 46 at rest, p = 0.74; 65 ± 56 vs. 37 ± 56 during pacing, p = 0.23).
CONCLUSIONS
Bone marrow cells secrete angiogenic factors that induce endothelial cell proliferation and, when injected transendocardially, augment collateral perfusion and myocardial function in ischemic myocardium.
|
Abbreviations and Acronyms
| | ABM | = autologous bone marrow | | BM | = bone marrow | | H&E | = hematoxylin and eosim | | LTCM | = long-term culture medium | | MCP-1 | = macrophage chemoattractant protein-1 | | PAEC | = pig aortic endothelial cells | | VEGF | = vascular endothelial growth factor |
|
This article has been cited by other articles:
|
|
|
|
 
M. Yousef, C. M. Schannwell, M. Kostering, T. Zeus, M. Brehm, and B. E. Strauer
The BALANCE Study: clinical benefit and long-term outcome after intracoronary autologous bone marrow cell transplantation in patients with acute myocardial infarction.
J. Am. Coll. Cardiol.,
June 16, 2009;
53(24):
2262 - 2269.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. van Ramshorst, J. J. Bax, S. L. M. A. Beeres, P. Dibbets-Schneider, S. D. Roes, M. P. M. Stokkel, A. de Roos, W. E. Fibbe, J. J. Zwaginga, E. Boersma, et al.
Intramyocardial Bone Marrow Cell Injection for Chronic Myocardial Ischemia: A Randomized Controlled Trial
JAMA,
May 20, 2009;
301(19):
1997 - 2004.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Mollmann, H. Nef, A. Elsasser, and C. Hamm
Stem cells in myocardial infarction: from bench to bedside
Heart,
March 1, 2009;
95(6):
508 - 514.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J van Ramshorst, D E Atsma, S L M A Beeres, S A Mollema, N Ajmone Marsan, E R Holman, E E van der Wall, M J Schalij, and J J Bax
Effect of intramyocardial bone marrow cell injection on left ventricular dyssynchrony and global strain
Heart,
January 15, 2009;
95(2):
119 - 124.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. E.A. van der Bogt, A. Y. Sheikh, S. Schrepfer, G. Hoyt, F. Cao, K. J. Ransohoff, R.-J. Swijnenburg, J. Pearl, A. Lee, M. Fischbein, et al.
Comparison of Different Adult Stem Cell Types for Treatment of Myocardial Ischemia
Circulation,
September 30, 2008;
118(14_suppl_1):
S121 - S129.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Psaltis, A. C.W. Zannettino, S. G. Worthley, and S. Gronthos
Concise Review: Mesenchymal Stromal Cells: Potential for Cardiovascular Repair
Stem Cells,
September 1, 2008;
26(9):
2201 - 2210.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. de Silva, A. N. Raval, M. Hadi, K. M. Gildea, A. C. Bonifacino, Z.-X. Yu, Y. Y. Yau, S. F. Leitman, S. L. Bacharach, R. E. Donahue, et al.
Intracoronary infusion of autologous mononuclear cells from bone marrow or granulocyte colony-stimulating factor-mobilized apheresis product may not improve remodelling, contractile function, perfusion, or infarct size in a swine model of large myocardial infarction
Eur. Heart J.,
July 2, 2008;
29(14):
1772 - 1782.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Sun, J. Wu, H. Fujii, J. Wu, S.-H. Li, S. Porozov, A. Belleli, V. Fulga, Y. Porat, and R.-K. Li
Human angiogenic cell precursors restore function in the infarcted rat heart: A comparison of cell delivery routes
Eur J Heart Fail,
June 1, 2008;
10(6):
525 - 533.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Horvath and Y. Zhou
Transmyocardial Laser Revascularization and Extravascular Angiogenetic Techniques to Increase Myocardial Blood Flow
Card. Surg. Adult,
January 1, 2008;
3(2008):
733 - 752.
[Full Text]
|
|
|
|
|
|
|
H.-F. Tse, C.-W. Siu, S.-G. Zhu, L. Songyan, Q.-Y. Zhang, W.-H. Lai, Y.-L. Kwong, J. Nicholls, and C.-P. Lau
Paracrine effects of direct intramyocardial implantation of bone marrow derived cells to enhance neovascularization in chronic ischaemic myocardium
Eur J Heart Fail,
August 1, 2007;
9(8):
747 - 753.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Song, W. Chang, S. Lim, H.-S. Seo, C. Y. Shim, S. Park, K.-J. Yoo, B.-S. Kim, B.-H. Min, H. Lee, et al.
Tissue Transglutaminase Is Essential for Integrin-Mediated Survival of Bone Marrow-Derived Mesenchymal Stem Cells
Stem Cells,
June 1, 2007;
25(6):
1431 - 1438.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Zbinden, L. C. Clavijo, B. Kantor, H. Morsli, G. A. Cortes, J. A. Andrews, G. J. Jang, M. S. Burnett, and S. E. Epstein
Interanimal variability in preexisting collaterals is a major factor determining outcome in experimental angiogenesis trials
Am J Physiol Heart Circ Physiol,
April 1, 2007;
292(4):
H1891 - H1897.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S L M A Beeres, J J Bax, K Zeppenfeld, P Dibbets-Schneider, M P M Stokkel, W E Fibbe, E E van der Wall, M J Schalij, and D E Atsma
Feasibility of trans-endocardial cell transplantation in chronic ischaemia
Heart,
January 1, 2007;
93(1):
113 - 114.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. T. Wall, J. C. Walker, K. E. Healy, M. B. Ratcliffe, and J. M. Guccione
Theoretical Impact of the Injection of Material Into the Myocardium: A Finite Element Model Simulation
Circulation,
December 12, 2006;
114(24):
2627 - 2635.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Assmus, D. H. Walter, R. Lehmann, J. Honold, H. Martin, S. Dimmeler, A. M. Zeiher, and V. Schachinger
Intracoronary infusion of progenitor cells is not associated with aggravated restenosis development or atherosclerotic disease progression in patients with acute myocardial infarction
Eur. Heart J.,
December 2, 2006;
27(24):
2989 - 2995.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. D. Moelker, T. Baks, E.J. van den Bos, R.J. van Geuns, P.J. de Feyter, D. J. Duncker, and W.J. van der Giessen
Reduction in infarct size, but no functional improvement after bone marrow cell administration in a porcine model of reperfused myocardial infarction
Eur. Heart J.,
December 2, 2006;
27(24):
3057 - 3064.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Boodhwani, S. Mieno, P. Voisine, J. Feng, N. Sodha, J. Li, and F. W. Sellke
High-dose atorvastatin is associated with impaired myocardial angiogenesis in response to vascular endothelial growth factor in hypercholesterolemic swine
J. Thorac. Cardiovasc. Surg.,
December 1, 2006;
132(6):
1299 - 1306.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kawamoto, H. Iwasaki, K. Kusano, T. Murayama, A. Oyamada, M. Silver, C. Hulbert, M. Gavin, A. Hanley, H. Ma, et al.
CD34-Positive Cells Exhibit Increased Potency and Safety for Therapeutic Neovascularization After Myocardial Infarction Compared With Total Mononuclear Cells
Circulation,
November 14, 2006;
114(20):
2163 - 2169.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. G. Shaffer, S. Greene, A. Arshi, G. Supple, A. Bantly, J. S Moores, M. S Parmacek, and E. R Mohler III
Effect of acute exercise on endothelial progenitor cells in patients with peripheral arterial disease
Vascular Medicine,
November 1, 2006;
11(4):
219 - 226.
[Abstract]
[PDF]
|
|
|
|
|
|
|
C. Kubal, K. Sheth, B. Nadal-Ginard, and M. Galinanes
Bone marrow cells have a potent anti-ischemic effect against myocardial cell death in humans.
J. Thorac. Cardiovasc. Surg.,
November 1, 2006;
132(5):
1112 - 1118.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Ben-Dor, S. Fuchs, and R. Kornowski
Potential Hazards and Technical Considerations Associated With Myocardial Cell Transplantation Protocols for Ischemic Myocardial Syndrome
J. Am. Coll. Cardiol.,
October 17, 2006;
48(8):
1519 - 1526.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Lunde, S. Solheim, S. Aakhus, H. Arnesen, M. Abdelnoor, T. Egeland, K. Endresen, A. Ilebekk, A. Mangschau, J. G. Fjeld, et al.
Intracoronary injection of mononuclear bone marrow cells in acute myocardial infarction.
N. Engl. J. Med.,
September 21, 2006;
355(12):
1199 - 1209.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Saeed, A. J. Martin, R. J. Lee, O. Weber, D. Revel, D. Saloner, and C. B. Higgins
MR Guidance of Targeted Injections into Border and Core of Scarred Myocardium in Pigs
Radiology,
August 1, 2006;
240(2):
419 - 426.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Boodhwani, Y. Nakai, P. Voisine, J. Feng, J. Li, S. Mieno, B. Ramlawi, C. Bianchi, R. Laham, and F. W. Sellke
High-Dose Atorvastatin Improves Hypercholesterolemic Coronary Endothelial Dysfunction Without Improving the Angiogenic Response
Circulation,
July 4, 2006;
114(1_suppl):
I-402 - I-408.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Kaigler, P.H. Krebsbach, Z. Wang, E.R. West, K. Horger, and D.J. Mooney
Transplanted Endothelial Cells Enhance Orthotopic Bone Regeneration
Journal of Dental Research,
July 1, 2006;
85(7):
633 - 637.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Hasegawa, H. Takano, K. Iwanaga, M. Ohtsuka, Y. Qin, Y. Niitsuma, K. Ueda, T. Toyoda, H. Tadokoro, and I. Komuro
Cardioprotective Effects of Granulocyte Colony-Stimulating Factor in Swine With Chronic Myocardial Ischemia
J. Am. Coll. Cardiol.,
February 21, 2006;
47(4):
842 - 849.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Ye, H. K. Haider, and E. K. W. Sim
Adult Stem Cells for Cardiac Repair: A Choice Between Skeletal Myoblasts and Bone Marrow Stem Cells
Experimental Biology and Medicine,
January 1, 2006;
231(1):
8 - 19.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Dimarakis, N. A. Habib, and M. Y.A. Gordon
Adult bone marrow-derived stem cells and the injured heart: just the beginning?
Eur. J. Cardiothorac. Surg.,
November 1, 2005;
28(5):
665 - 676.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Sartore, M. Lenzi, A. Angelini, A. Chiavegato, L. Gasparotto, P. D. Coppi, R. Bianco, and G. Gerosa
Amniotic mesenchymal cells autotransplanted in a porcine model of cardiac ischemia do not differentiate to cardiogenic phenotypes
Eur. J. Cardiothorac. Surg.,
November 1, 2005;
28(5):
677 - 684.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. E. Strauer, M. Brehm, T. Zeus, T. Bartsch, C. Schannwell, C. Antke, R. V. Sorg, G. Kogler, P. Wernet, H.-W. Muller, et al.
Regeneration of Human Infarcted Heart Muscle by Intracoronary Autologous Bone Marrow Cell Transplantation in Chronic Coronary Artery Disease: The IACT Study
J. Am. Coll. Cardiol.,
November 1, 2005;
46(9):
1651 - 1658.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. R. Makkar, M. J. Price, M. Lill, M. Frantzen, K. Takizawa, T. Kleisli, J. Zheng, S. Kar, R. McClelan, T. Miyamota, et al.
Intramyocardial Injection of Allogenic Bone Marrow-Derived Mesenchymal Stem Cells Without Immunosuppression Preserves Cardiac Function in a Porcine Model of Myocardial Infarction
Journal of Cardiovascular Pharmacology and Therapeutics,
October 1, 2005;
10(4):
225 - 233.
[Abstract]
[PDF]
|
|
|
|
|
|
|
L. Ye, H. Kh. Haider, S. Jiang, L. H. Ling, R. Ge, P. K. Law, and E. K.W. Sim
Reversal of myocardial injury using genetically modulated human skeletal myoblasts in a rodent cryoinjured heart model
Eur J Heart Fail,
October 1, 2005;
7(6):
945 - 952.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. J. Dzau, M. Gnecchi, A. S. Pachori, F. Morello, and L. G. Melo
Therapeutic Potential of Endothelial Progenitor Cells in Cardiovascular Diseases
Hypertension,
July 1, 2005;
46(1):
7 - 18.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. K. Haider and M. Ashraf
Bone marrow stem cell transplantation for cardiac repair
Am J Physiol Heart Circ Physiol,
June 1, 2005;
288(6):
H2557 - H2567.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Schachinger and A. M. Zeiher
Stem Cells and Cardiovascular and Renal Disease: Today and Tomorrow
J. Am. Soc. Nephrol.,
March 1, 2005;
16(3_suppl_1):
S2 - S6.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. C. Wollert and H. Drexler
Clinical Applications of Stem Cells for the Heart
Circ. Res.,
February 4, 2005;
96(2):
151 - 163.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. V. Silva, S. Litovsky, J. A.R. Assad, A. L.S. Sousa, B. J. Martin, D. Vela, S. C. Coulter, J. Lin, J. Ober, W. K. Vaughn, et al.
Mesenchymal Stem Cells Differentiate into an Endothelial Phenotype, Enhance Vascular Density, and Improve Heart Function in a Canine Chronic Ischemia Model
Circulation,
January 18, 2005;
111(2):
150 - 156.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Schachinger, B. Assmus, M. B. Britten, J. Honold, R. Lehmann, C. Teupe, N. D. Abolmaali, T. J. Vogl, W.-K. Hofmann, H. Martin, et al.
Transplantation of progenitor cells and regeneration enhancement in acute myocardial infarction: Final one-year results of the TOPCARE-AMI Trial
J. Am. Coll. Cardiol.,
October 19, 2004;
44(8):
1690 - 1699.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. G. Melo, M. Gnecchi, A. S. Pachori, D. Kong, K. Wang, X. Liu, R. E. Pratt, and V. J. Dzau
Endothelium-Targeted Gene and Cell-Based Therapies for Cardiovascular Disease
Arterioscler. Thromb. Vasc. Biol.,
October 1, 2004;
24(10):
1761 - 1774.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kawamoto, T. Murayama, K. Kusano, M. Ii, T. Tkebuchava, S. Shintani, A. Iwakura, I. Johnson, P. von Samson, A. Hanley, et al.
Synergistic Effect of Bone Marrow Mobilization and Vascular Endothelial Growth Factor-2 Gene Therapy in Myocardial Ischemia
Circulation,
September 14, 2004;
110(11):
1398 - 1405.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. C. Perin, H. F.R. Dohmann, R. Borojevic, S. A. Silva, A. L.S. Sousa, G. V. Silva, C. T. Mesquita, L. Belem, W. K. Vaughn, F. O.D. Rangel, et al.
Improved Exercise Capacity and Ischemia 6 and 12 Months After Transendocardial Injection of Autologous Bone Marrow Mononuclear Cells for Ischemic Cardiomyopathy
Circulation,
September 14, 2004;
110(11_suppl_1):
II-213 - II-218.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Heil and W. Schaper
Influence of Mechanical, Cellular, and Molecular Factors on Collateral Artery Growth (Arteriogenesis)
Circ. Res.,
September 3, 2004;
95(5):
449 - 458.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L.G Melo, M Gnecchi, A.S Pachori, K Wang, and V.J Dzau
Gene- and cell-based therapies for cardiovascular diseases: current status and future directions
Eur. Heart J. Suppl.,
September 1, 2004;
6(suppl_E):
E24 - E35.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Kinnaird, E. Stabile, M. S. Burnett, and S. E. Epstein
Bone Marrow-Derived Cells for Enhancing Collateral Development: Mechanisms, Animal Data, and Initial Clinical Experiences
Circ. Res.,
August 20, 2004;
95(4):
354 - 363.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. L. Olivares, V. P. Ribeiro, J. P. S. Werneck de Castro, K. C. Ribeiro, E. C. Mattos, R. C. S. Goldenberg, J. G. Mill, H. F. Dohmann, R. R. dos Santos, A. C. C. de Carvalho, et al.
Bone marrow stromal cells improve cardiac performance in healed infarcted rat hearts
Am J Physiol Heart Circ Physiol,
August 1, 2004;
287(2):
H464 - H470.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. F. Pittenger and B. J. Martin
Mesenchymal Stem Cells and Their Potential as Cardiac Therapeutics
Circ. Res.,
July 9, 2004;
95(1):
9 - 20.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-S. Yoon, J.-S. Park, T. Tkebuchava, C. Luedeman, and D. W. Losordo
Unexpected Severe Calcification After Transplantation of Bone Marrow Cells in Acute Myocardial Infarction
Circulation,
June 29, 2004;
109(25):
3154 - 3157.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. Epstein, E. Stabile, T. Kinnaird, C. W. Lee, L. Clavijo, and M. S. Burnett
Janus Phenomenon: The Interrelated Tradeoffs Inherent in Therapies Designed to Enhance Collateral Formation and Those Designed to Inhibit Atherogenesis
Circulation,
June 15, 2004;
109(23):
2826 - 2831.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. S. Lee and R. R. Makkar
Stem-Cell Transplantation in Myocardial Infarction: A Status Report
Ann Intern Med,
May 4, 2004;
140(9):
729 - 737.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Heeschen, R. Lehmann, J. Honold, B. Assmus, A. Aicher, D. H. Walter, H. Martin, A. M. Zeiher, and S. Dimmeler
Profoundly Reduced Neovascularization Capacity of Bone Marrow Mononuclear Cells Derived From Patients With Chronic Ischemic Heart Disease
Circulation,
April 6, 2004;
109(13):
1615 - 1622.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kinnaird, E. Stabile, M.S. Burnett, M. Shou, C.W. Lee, S. Barr, S. Fuchs, and S.E. Epstein
Local Delivery of Marrow-Derived Stromal Cells Augments Collateral Perfusion Through Paracrine Mechanisms
Circulation,
March 30, 2004;
109(12):
1543 - 1549.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kinnaird, E. Stabile, M.S. Burnett, C.W. Lee, S. Barr, S. Fuchs, and S.E. Epstein
Marrow-Derived Stromal Cells Express Genes Encoding a Broad Spectrum of Arteriogenic Cytokines and Promote In Vitro and In Vivo Arteriogenesis Through Paracrine Mechanisms
Circ. Res.,
March 19, 2004;
94(5):
678 - 685.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Rehman, D. Traktuev, J. Li, S. Merfeld-Clauss, C. J. Temm-Grove, J. E. Bovenkerk, C. L. Pell, B. H. Johnstone, R. V. Considine, and K. L. March
Secretion of Angiogenic and Antiapoptotic Factors by Human Adipose Stromal Cells
Circulation,
March 16, 2004;
109(10):
1292 - 1298.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Chachques, C. Acar, J. Herreros, J. C. Trainini, F. Prosper, N. D'Attellis, J.-N. Fabiani, and A. F. Carpentier
Cellular cardiomyoplasty: clinical application
Ann. Thorac. Surg.,
March 1, 2004;
77(3):
1121 - 1130.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ziegelhoeffer, B. Fernandez, S. Kostin, M. Heil, R. Voswinckel, A. Helisch, and W. Schaper
Bone Marrow-Derived Cells Do Not Incorporate Into the Adult Growing Vasculature
Circ. Res.,
February 6, 2004;
94(2):
230 - 238.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.B. Britten, N.D. Abolmaali, B. Assmus, R. Lehmann, J. Honold, J. Schmitt, T.J. Vogl, H. Martin, V. Schachinger, S. Dimmeler, et al.
Infarct Remodeling After Intracoronary Progenitor Cell Treatment in Patients With Acute Myocardial Infarction (TOPCARE-AMI): Mechanistic Insights From Serial Contrast-Enhanced Magnetic Resonance Imaging
Circulation,
November 4, 2003;
108(18):
2212 - 2218.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A.U. Heilmann, T. Attmann, A. Thiem, E. Haffner, F. Beyersdorf, and G. Lutter
Gene therapy in cardiac surgery: intramyocardial injection of naked plasmid DNA for chronic myocardial ischemia
Eur. J. Cardiothorac. Surg.,
November 1, 2003;
24(5):
785 - 793.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. S. Forrester, M. J. Price, and R. R. Makkar
Stem Cell Repair of Infarcted Myocardium: An Overview for Clinicians
Circulation,
September 2, 2003;
108(9):
1139 - 1145.
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Chhokar and A. L. Tucker
Angiogenesis: Basic Mechanisms and Clinical Applications
Seminars in Cardiothoracic and Vascular Anesthesia,
September 1, 2003;
7(3):
253 - 280.
[Abstract]
[PDF]
|
|
|
|
|
|
|
T.-S. Li, K. Hamano, M. Nishida, M. Hayashi, H. Ito, A. Mikamo, and M. Matsuzaki
CD117+ stem cells play a key role in therapeutic angiogenesis induced by bone marrow cell implantation
Am J Physiol Heart Circ Physiol,
August 7, 2003;
285(3):
H931 - H937.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A. Thompson, B. A. Nasseri, J. Makower, S. Houser, M. McGarry, T. Lamson, I. Pomerantseva, J. Y. Chang, H. K. Gold, J. P. Vacanti, et al.
Percutaneous transvenous cellular cardiomyoplasty: A novel nonsurgical approach for myocardial cell transplantation
J. Am. Coll. Cardiol.,
June 4, 2003;
41(11):
1964 - 1971.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. H.L. Tang, P. W.M. Fedak, T. M. Yau, R. D. Weisel, A. Kulik, D. A.G. Mickle, and R.-K. Li
Cell transplantation to improve ventricular function in the failing heart
Eur. J. Cardiothorac. Surg.,
June 1, 2003;
23(6):
907 - 916.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Fuchs, L. F. Satler, R. Kornowski, P. Okubagzi, G. Weisz, R. Baffour, R. Waksman, N. J. Weissman, M. Cerqueira, M. B. Leon, et al.
Catheter-based autologous bone marrow myocardial injection in no-option patients with advanced coronary artery disease: A feasibility study
J. Am. Coll. Cardiol.,
May 21, 2003;
41(10):
1721 - 1724.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.e. Garot, T. Unterseeh, E. Teiger, S. Champagne, B.e. Chazaud, R. Gherardi, L. Hittinger, P. Gueret, and A. Rahmouni
Magnetic resonance imaging of targeted catheter-based implantation of myogenic precursor cells into infarcted left ventricular myocardium
J. Am. Coll. Cardiol.,
May 21, 2003;
41(10):
1841 - 1846.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. C. Perin, H. F.R. Dohmann, R. Borojevic, S. A. Silva, A. L.S. Sousa, C. T. Mesquita, M. I.D. Rossi, A. C. Carvalho, H. S. Dutra, H. J.F. Dohmann, et al.
Transendocardial, Autologous Bone Marrow Cell Transplantation for Severe, Chronic Ischemic Heart Failure
Circulation,
May 13, 2003;
107(18):
2294 - 2302.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. C. Hughes, M. J. Post, M. Simons, and B. H. Annex
Translational Physiology: Porcine models of human coronary artery disease: implications for preclinical trials of therapeutic angiogenesis
J Appl Physiol,
May 1, 2003;
94(5):
1689 - 1701.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Luttun and P. Carmeliet
De novo vasculogenesis in the heart
Cardiovasc Res,
May 1, 2003;
58(2):
378 - 389.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Masuda and T. Asahara
Post-natal endothelial progenitor cells for neovascularization in tissue regeneration
Cardiovasc Res,
May 1, 2003;
58(2):
390 - 398.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y Schwartz and R Kornowski
Progenitor and embryonic stem cell transplantation for myocardial angiogenesis and functional restoration
Eur. Heart J.,
March 1, 2003;
24(5):
404 - 411.
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. E. Strauer and R. Kornowski
Stem Cell Therapy in Perspective
Circulation,
February 25, 2003;
107(7):
929 - 934.
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. C. Perin, Y.-J. Geng, and J. T. Willerson
Adult Stem Cell Therapy in Perspective
Circulation,
February 25, 2003;
107(7):
935 - 938.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kawamoto, T. Tkebuchava, J.-I. Yamaguchi, H. Nishimura, Y.-S. Yoon, C. Milliken, S. Uchida, O. Masuo, H. Iwaguro, H. Ma, et al.
Intramyocardial Transplantation of Autologous Endothelial Progenitor Cells for Therapeutic Neovascularization of Myocardial Ischemia
Circulation,
January 28, 2003;
107(3):
461 - 468.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ruel, R. A. Kelly, and F. W. Sellke
Therapeutic Angiogenesis, Transmyocardial Laser Revascularization, and Cell Therapy
Card. Surg. Adult,
January 1, 2003;
2(2003):
715 - 750.
[Full Text]
|
|
|
|
|
|
|
B. Assmus, V. Schachinger, C. Teupe, M. Britten, R. Lehmann, N. Dobert, F. Grunwald, A. Aicher, C. Urbich, H. Martin, et al.
Transplantation of Progenitor Cells and Regeneration Enhancement in Acute Myocardial Infarction (TOPCARE-AMI)
Circulation,
December 10, 2002;
106(24):
3009 - 3017.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kamihata, H. Matsubara, T. Nishiue, S. Fujiyama, K. Amano, O. Iba, T. Imada, and T. Iwasaka
Improvement of Collateral Perfusion and Regional Function by Implantation of Peripheral Blood Mononuclear Cells Into Ischemic Hibernating Myocardium
Arterioscler. Thromb. Vasc. Biol.,
November 1, 2002;
22(11):
1804 - 1810.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. C. Perin, G. V. Silva, R. Sarmento-Leite, A. L.S. Sousa, M. Howell, R. Muthupillai, B. Lambert, W. K. Vaughn, and S. D. Flamm
Assessing Myocardial Viability and Infarct Transmurality With Left Ventricular Electromechanical Mapping in Patients With Stable Coronary Artery Disease: Validation by Delayed-Enhancement Magnetic Resonance Imaging
Circulation,
August 20, 2002;
106(8):
957 - 961.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
