EXPERIMENTAL STUDY
Atherosclerotic aortic component quantification by noninvasive magnetic resonance imaging: an in vivo study in rabbits
G.érard Helft, MD, PhD*  ,
Stephen G. Worthley, MBBS, FRACP* ,
Valentin Fuster, MD, PhD, FACC,
Azfar G. Zaman, MD, MRCP* ,
Clyde Schechter, MD ,
Julio I. Osende, MD* ,
Oswaldo J. Rodriguez, MD* ,
Zahi A. Fayad, PhD,
John T. Fallon, MD, PhD  and
Juan J. Badimon, PhD, FACC*
* Cardiovascular Biology Research Laboratory, New York, New York, USA
Zena and Michael A. Wiener Cardiovascular Institute, New York, New York, USA
Department of Pathology, Mount Sinai Medical Center, New York, New York, USA
Department of Family Medicine and Community Health, Albert Einstein College of Medicine, New York, New York, USA
Manuscript received December 20, 1999;
revised manuscript received November 3, 2000,
accepted December 12, 2000.
Reprint requests and correspondence: Dr. Juan J. Badimon, Cardiovascular Biology Research Laboratory, Zena and Michael A. Wiener Cardiovascular Institute, Mount Sinai School of Medicine, New York, New York 10029-6574
juan.badimon{at}mssm.edu
OBJECTIVES
We sought to demonstrate the ability that noninvasive in vivo magnetic resonance imaging (MRI) has to quantify the different components within atherosclerotic plaque.
BACKGROUND
Atherosclerotic plaque composition plays a critical role in both lesion stability and subsequent thrombogenicity. Noninvasive MRI is a promising tool for the characterization of plaque composition.
METHODS
Thoracic and abdominal aortic atherosclerotic lesions were induced in rabbits (n = 5). Nine months later, MRI was performed in a 1.5T system. Fast spin-echo sequences (proton density-weighted and T2-weighted [T2W] images) were obtained (in-plane resolution: 350 x 350 microns, slice thickness: 3 mm). Magnetic resonance images were correlated with matched histopathological sections (n = 108).
RESULTS
A significant correlation (p < 0.001) was observed for mean wall thickness and vessel wall area between MRI and histopathology (r = 0.87 and r = 0.85, respectively). The correlation was also present on subanalysis of the thoracic and upper part of the abdominal aorta, susceptible to respiratory motion artifacts. There was a significant correlation for plaque composition (p < 0.05) between MRI and histopathology for the analysis of lipidic (low signal on T2W, r = 0.81) and fibrous (high signal on T2W, r = 0.86) areas with Oil Red O staining. T2-weighted images showed greater contrast than proton density-weighted between these different components of the plaques as assessed by signal intensity ratio analysis with the mean difference in signal ratios of 0.47 (S.E. 0.012, adjusted for clustering of observations within lesions) being significantly different from 0 (t1 = 39.1, p = 0.016).
CONCLUSIONS
In vivo noninvasive high resolution MRI accurately quantifies fibrotic and lipidic components of atherosclerosis in this model. This may permit the serial analysis of therapeutic strategies on atherosclerotic plaque stabilization.
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Abbreviations and Acronyms
| | MR | = magnetic resonance | | MRI | = magnetic resonance imaging | | MWT | = mean wall thickness | | PDW | = proton density-weighted | | T2W | = T2-weighted | | VWA | = vessel wall area |
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