|
|
||||||||||
|
J Am Coll Cardiol, 1999; 34:1420-1426 © 1999 by the American College of Cardiology Foundation |
a Deutsches Herzzentrum and 1. Medizinische Klinik der Technischen Universität München, Munich, Germany
Manuscript received January 14, 1999; revised manuscript received May 20, 1999, accepted June 28, 1999.
Reprint requests and correspondence: Dr. Franz-Josef Neumann, Deutsches Herzzentrum an der Technischen Universität, Lazarettstr. 36, 81636 München, Germany.
neumann{at}dhm.mhn.de
OBJECTIVES
This prospective randomized study investigated platelet-induced upregulation of Mac-1 on monocytes and its inhibition by glycoprotein (GP) IIb/IIIa blockage in patients with acute myocardial infarction (AMI).
BACKGROUND
In experimental AMI, Mac-1 on leukocytes is the pivotal adhesion molecule for detrimental inflammatory responses. In vitro, platelet adhesion to monocytes upregulates Mac-1.
METHODS
Patients undergoing stenting in AMI within 48 h after onset of symptoms were randomly assigned to receive either standard-dose heparin (n = 50) or abciximab plus low-dose heparin (n = 50). In serial blood samples, we assessed platelet-monocyte interaction and Mac-1 surface expression by triple color immunofluorescence flow cytometry.
RESULTS
Compared with platelet-negative monocytes, Mac-1 surface expression on monocytes with attached platelets was upregulated (median fluorescence intensity [interquartile range]: 259 [179 to 367] vs. 135 [78 to 195] arbitrary units, p < 0.001). As an indicator of platelet-monocyte interaction, mean fluorescence of the platelet marker GP Ib
in the monocytes population decreased after abciximab, although it remained unaffected by heparin alone. Abciximab achieved this effect by a reduction in platelet mass attached to monocytes (GP Ib
fluorescence intensity of heterotypic aggregates at 24 h [arbitrary units]: 187 [143 to 236] after abciximab vs. 228 [156 to 332] after heparin, p = 0.02), whereas it did not affect the percentage of monocytes with adherent platelets. Reduction of platelet-monocyte interaction resulted in decreased Mac-1 surface expression (fluorescence intensity at 24 h [arbitrary units]: 116 [68 to 153] after abciximab vs. 162 [117 to 239] after heparin, p = 0.001).
CONCLUSIONS
In patients with AMI, platelet-leukocyte interactions modulate Mac-1 expression on monocytes. Glycoprotein IIb/IIIa blockade is a therapeutic option to interfere with this mechanism.
| ||||||||||||||||
This article has been cited by other articles:
![]() |
A. E. May, P. Seizer, and M. Gawaz Platelets: Inflammatory Firebugs of Vascular Walls Arterioscler. Thromb. Vasc. Biol., March 1, 2008; 28(3): s5 - s10. [Abstract] [Full Text] [PDF] |
||||
![]() |
W. Hochholzer, D. Schlittenhardt, T. Arentz, J. Stockinger, R. Weber, G. Burkle, D. Kalusche, D. Trenk, and F.-J. Neumann Platelet activation and myocardial necrosis in patients undergoing radiofrequency and cryoablation of isthmus-dependent atrial flutter Europace, July 1, 2007; 9(7): 490 - 495. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. R. Steinhubl, J. J. Badimon, D. L. Bhatt, J.-M. Herbert, and T. F. Luscher Clinical evidence for anti-inflammatory effects of antiplatelet therapy in patients with atherothrombotic disease Vascular Medicine, May 1, 2007; 12(2): 113 - 122. [Abstract] [PDF] |
||||
![]() |
M. Sezer, I. Okcular, T. Goren, H. Oflaz, Y. Nisanci, B. Umman, F. Mercanoglu, A. K Bilge, M. Meric, and S. Umman Association of haematological indices with the degree of microvascular injury in patients with acute anterior wall myocardial infarction treated with primary percutaneous coronary intervention Heart, March 1, 2007; 93(3): 313 - 318. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. Antoniucci Differences among GP IIb/IIIa inhibitors: different clinical benefits in non-ST-segment elevation acute coronary syndrome percutaneous coronary intervention patients Eur. Heart J. Suppl., February 1, 2007; 9(suppl_A): A32 - A36. [Abstract] [Full Text] [PDF] |
||||
![]() |
S A Harding, J N Din, J Sarma, D H Josephs, K A A Fox, and D E Newby Promotion of proinflammatory interactions between platelets and monocytes by unfractionated heparin Heart, November 1, 2006; 92(11): 1635 - 1638. [Abstract] [Full Text] [PDF] |
||||
![]() |
T Heer, U Zeymer, C Juenger, A K Gitt, H Wienbergen, R Zahn, M Gottwik, J Senges, and for the Acute Coronary Syndromes (ACOS) Registry I Beneficial effects of abciximab in patients with primary percutaneous intervention for acute ST segment elevation myocardial infarction in clinical practice Heart, October 1, 2006; 92(10): 1484 - 1489. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. Htun, S. Fateh-Moghadam, B. Tomandl, R. Handschu, K. Klinger, K. Stellos, C. Garlichs, W. Daniel, and M. Gawaz Course of Platelet Activation and Platelet-Leukocyte Interaction in Cerebrovascular Ischemia Stroke, September 1, 2006; 37(9): 2283 - 2287. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. E. Freedman Molecular Regulation of Platelet-Dependent Thrombosis Circulation, October 25, 2005; 112(17): 2725 - 2734. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Ferenc and F.-J. Neumann Efficacy of primary PCI: the microvessel perspective Eur. Heart J. Suppl., October 1, 2005; 7(suppl_I): I4 - I9. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Migliorini and D. Antoniucci Patient selection bias in primary percutaneous coronary intervention trials: a critical issue Eur. Heart J. Suppl., October 1, 2005; 7(suppl_I): I21 - I26. [Abstract] [Full Text] [PDF] |
||||
![]() |
Z. Huczek, J. Kochman, K. J. Filipiak, G. J. Horszczaruk, M. Grabowski, R. Piatkowski, J. Wilczynska, A. Zielinski, B. Meier, and G. Opolski Mean Platelet Volume on Admission Predicts Impaired Reperfusion and Long-Term Mortality in Acute Myocardial Infarction Treated With Primary Percutaneous Coronary Intervention J. Am. Coll. Cardiol., July 19, 2005; 46(2): 284 - 290. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. A. Chirinos, G. A. Heresi, H. Velasquez, W. Jy, J. J. Jimenez, E. Ahn, L. L. Horstman, A. O. Soriano, J. P. Zambrano, and Y. S. Ahn Elevation of Endothelial Microparticles, Platelets, and Leukocyte Activation in Patients With Venous Thromboembolism J. Am. Coll. Cardiol., May 3, 2005; 45(9): 1467 - 1471. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. Sciagra, G. Parodi, A. Pupi, A. Migliorini, R. Valenti, G. Moschi, G. M. Santoro, G. Memisha, and D. Antoniucci Gated SPECT Evaluation of Outcome After Abciximab-Supported Primary Infarct Artery Stenting for Acute Myocardial Infarction: The Scintigraphic Data of the Abciximab and Carbostent Evaluation (ACE) Randomized Trial J. Nucl. Med., May 1, 2005; 46(5): 722 - 727. [Abstract] [Full Text] [PDF] |
||||
![]() |
L. S. Ritter, K. M. Stempel, B. M. Coull, and P. F. McDonagh Leukocyte-Platelet Aggregates in Rat Peripheral Blood After Ischemic Stroke and Reperfusion Biol Res Nurs, April 1, 2005; 6(4): 281 - 288. [Abstract] [PDF] |
||||
![]() |
W.H. W. Tang and A. M. Lincoff Diabetes, Coronary Intervention, and Platelet Glycoprotein IIb/IIIa Blockade: The Triad Revisited Circulation, December 14, 2004; 110(24): 3618 - 3620. [Full Text] [PDF] |
||||
![]() |
T. Inoue, T. Uchida, M. Sakuma, Y. Imoto, Y. Ozeki, Y. Ozaki, Y. Hikichi, and K. Node Cilostazol inhibits leukocyte integrin Mac-1, leading to a potential reduction in restenosis after coronary stent implantation J. Am. Coll. Cardiol., October 6, 2004; 44(7): 1408 - 1414. [Abstract] [Full Text] [PDF] |
||||
![]() |
J A Zeller, K Frahm, R Baron, R Stingele, and G Deuschl Platelet-leukocyte interaction and platelet activation in migraine: a link to ischemic stroke? J. Neurol. Neurosurg. Psychiatry, July 1, 2004; 75(7): 984 - 987. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. D. Garlichs, S. Eskafi, I. Cicha, A. Schmeisser, B. Walzog, D. Raaz, C. Stumpf, A. Yilmaz, J. Bremer, J. Ludwig, et al. Delay of neutrophil apoptosis in acute coronary syndromes J. Leukoc. Biol., May 1, 2004; 75(5): 828 - 835. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Gawaz Role of platelets in coronary thrombosis and reperfusion of ischemic myocardium Cardiovasc Res, February 15, 2004; 61(3): 498 - 511. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. W. Kopp, S. Steiner, C. Nasel, D. Seidinger, I. Mlekusch, W. Lang, A. Bartok, R. Ahmadi, and E. Minar Abciximab Reduces Monocyte Tissue Factor in Carotid Angioplasty and Stenting Stroke, November 1, 2003; 34(11): 2560 - 2567. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Steiner, D. Seidinger, K. Huber, C. Kaun, E. Minar, and C. W. Kopp Effect of Glycoprotein IIb/IIIa Antagonist Abciximab on Monocyte-Platelet Aggregates and Tissue Factor Expression Arterioscler. Thromb. Vasc. Biol., September 1, 2003; 23(9): 1697 - 1702. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Heitzer, I. Ollmann, K. Koke, T. Meinertz, and T. Munzel Platelet Glycoprotein IIb/IIIa Receptor Blockade Improves Vascular Nitric Oxide Bioavailability in Patients With Coronary Artery Disease Circulation, August 5, 2003; 108(5): 536 - 541. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Dibra, J. Mehilli, M. Schwaiger, H. Schuhlen, H. Bollwein, S. Braun, J. Neverve, A. Schomig, and A. Kastrati Predictive value of basal C-reactive protein levels for myocardial salvage in patients with acute myocardial infarction is dependent on the type of reperfusion treatment Eur. Heart J., June 2, 2003; 24(12): 1128 - 1133. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. J. Brener, S. G. Ellis, J. Schneider, C. Apperson-Hansen, and E. J. Topol Abciximab-facilitated percutaneous coronary intervention and long-term survival--a prospective single-center registry Eur. Heart J., April 1, 2003; 24(7): 630 - 638. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Marzilli, G. Sambuceti, R. Testa, and S. Fedele Platelet glycoprotein IIb/IIIa receptor blockade and coronary resistance in unstable angina J. Am. Coll. Cardiol., December 18, 2002; 40(12): 2102 - 2109. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. S. Sabatine, D. A. Morrow, C. P. Cannon, S. A. Murphy, L. A. Demopoulos, P. M. DiBattiste, C. H. McCabe, E. Braunwald, and C. M. Gibson Relationship between baseline white blood cell count and degree of coronary artery disease and mortality in patients with acute coronary syndromes: A TACTICS-TIMI 18 substudy J. Am. Coll. Cardiol., November 20, 2002; 40(10): 1761 - 1768. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Kupatt, R. Wichels, J. Horstkotte, F. Krombach, H. Habazettl, and P. Boekstegers Molecular mechanisms of platelet-mediated leukocyte recruitment during myocardial reperfusion J. Leukoc. Biol., September 1, 2002; 72(3): 455 - 461. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Palmerini, M. A. Nedelman, L. E. Scudder, M. T. Nakada, R. E. Jordan, S. Smyth, R. E. Gordon, J. T. Fallon, and B. S. Coller Effects of abciximab on the acute pathology of blood vessels after arterial stenting in nonhuman primates J. Am. Coll. Cardiol., July 17, 2002; 40(2): 360 - 366. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. J. Quinn, E. F. Plow, and E. J. Topol Platelet Glycoprotein IIb/IIIa Inhibitors: Recognition of a Two-Edged Sword? Circulation, July 16, 2002; 106(3): 379 - 385. [Full Text] [PDF] |
||||
![]() |
G. W. Stone, D. J. Moliterno, M. Bertrand, F.-J. Neumann, H. C. Herrmann, E. R. Powers, C. L. Grines, J. W. Moses, D. J. Cohen, E. A. Cohen, et al. Impact of Clinical Syndrome Acuity on the Differential Response to 2 Glycoprotein IIb/IIIa Inhibitors in Patients Undergoing Coronary Stenting: The TARGET Trial* Circulation, May 21, 2002; 105(20): 2347 - 2354. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. E. Freedman and J. Loscalzo Platelet-Monocyte Aggregates: Bridging Thrombosis and Inflammation Circulation, May 7, 2002; 105(18): 2130 - 2132. [Full Text] [PDF] |
||||
![]() |
S.J. Brener and E.J. Topol Epicardial versus microcirculatory dissociation Eur. Heart J., February 2, 2002; 23(4): 274 - 276. [Full Text] [PDF] |
||||
![]() |
A. J. Taylor, A. Bobik, M. C. Berndt, D. Ramsay, and G. Jennings Experimental Rupture of Atherosclerotic Lesions Increases Distal Vascular Resistance: A Limiting Factor to the Success of Infarct Angioplasty Arterioscler. Thromb. Vasc. Biol., January 1, 2002; 22(1): 153 - 160. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. AUKRUST, T. WAeHRE, J. K. DAMAS, L. GULLESTAD, and N. O. SOLUM Inflammatory role of platelets in acute coronary syndromes Heart, December 1, 2001; 86(6): 605 - 606. [Full Text] [PDF] |
||||
![]() |
A. M. Lincoff, D. J. Kereiakes, M. A. Mascelli, L. I. Deckelbaum, E. S. Barnathan, K. K. Patel, B. Frederick, M. T. Nakada, and E. J. Topol Abciximab Suppresses the Rise in Levels of Circulating Inflammatory Markers After Percutaneous Coronary Revascularization Circulation, July 10, 2001; 104(2): 163 - 167. [Abstract] [Full Text] [PDF] |
||||
![]() |
E. J. Topol, D. J. Moliterno, H. C. Herrmann, E. R. Powers, C. L. Grines, D. J. Cohen, E. A. Cohen, M. Bertrand, F.-J. Neumann, G. W. Stone, et al. Comparison of Two Platelet Glycoprotein IIb/IIIa Inhibitors, Tirofiban and Abciximab, for the Prevention of Ischemic Events with Percutaneous Coronary Revascularization N. Engl. J. Med., June 21, 2001; 344(25): 1888 - 1894. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. W. Armstrong and D. Collen Fibrinolysis for Acute Myocardial Infarction : Current Status and New Horizons for Pharmacological Reperfusion, Part 2 Circulation, June 19, 2001; 103(24): 2987 - 2992. [Full Text] [PDF] |
||||
![]() |
E. Braunwald Foreword Eur. Heart J. Suppl., May 1, 2001; 3(suppl_A): A1 - A2. [PDF] |
||||
![]() |
F.-J. Neumann Optimization of microvascular reperfusion in acute myocardial infarction Eur. Heart J. Suppl., May 1, 2001; 3(suppl_A): A21 - A25. [Abstract] [PDF] |
||||
![]() |
F.-J. Neumann, W. Hochholzer, G. Pogatsa-Murray, A. Schomig, and M. Gawaz Antiplatelet effects of abciximab, tirofiban and eptifibatide in patients undergoing coronary stenting J. Am. Coll. Cardiol., April 1, 2001; 37(5): 1323 - 1328. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Kupatt, H. Habazettl, P. Hanusch, R. Wichels, D. Hahnel, B. F. Becker, and P. Boekstegers c7E3Fab Reduces Postischemic Leukocyte-Thrombocyte Interaction Mediated by Fibrinogen : Implications for Myocardial Reperfusion Injury Arterioscler. Thromb. Vasc. Biol., October 1, 2000; 20(10): 2226 - 2232. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Schomig, A. Kastrati, J. Dirschinger, J. Mehilli, U. Schricke, J. Pache, S. Martinoff, F.-J. Neumann, M. Schwaiger, and The Stent versus Thrombolysis for Occluded Coronar Coronary Stenting plus Platelet Glycoprotein IIb/IIIa Blockade Compared with Tissue Plasminogen Activator in Acute Myocardial Infarction N. Engl. J. Med., August 10, 2000; 343(6): 385 - 391. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. M. Smith and J. L. Mehta Reviews: Glycoprotein IIb/IIIa Receptor Inhibitors in Acute Coronary Syndromes: Conquests and New Challenges Journal of Cardiovascular Pharmacology and Therapeutics, January 1, 2000; 5(3): 143 - 150. [PDF] |
||||
![]() |
T. A. Waldmann, R. Levy, and B. S. Coller Emerging Therapies: Spectrum of Applications of Monoclonal Antibody Therapy Hematology, January 1, 2000; 2000(1): 394 - 408. [Abstract] [Full Text] [PDF] |
||||
| HOME | SUBSCRIPTIONS | CURRENT ISSUE | PAST ISSUES | CARDIOSOURCE | SEARCH | HELP | FEEDBACK |