CLINICAL STUDIES
Expression and distribution of brain natriuretic peptide in human right atria
Kiyoshi Doyama, MD*,
Manabu Fukumoto, MD*,
Genzou Takemura, MD ,
Masaru Tanaka, MD*,
Teiji Oda, MD ,
Koji Hasegawa, MD*,
Tsukasa Inada, MD*,
Seiji Ohtani, MD*,
Takako Fujiwara, MD ,
Hiroshi Itoh, MD*,
Kazuwa Nakao, MD*,
Shigetake Sasayama, MD* and
Hisayoshi Fujiwara, MD
* Third and Second Department of Internal Medicine and Second Department of Pathology, Kyoto University School of Medicine, Kyoto, Japan
Second Department of Internal Medicine, Gifu University School of Medicine, Gifu, Japan
Department of Cardiovascular Surgery, Rakuwakai-Otowa Hospital, Kyoto, Japan
Kyoto Women’s University, Kyoto, Japan
Manuscript received March 16, 1998;
revised manuscript received July 20, 1998,
accepted August 20, 1998.
Address for correspondence: Dr. Hisayoshi Fujiwara, Second Department of Internal Medicine, Gifu University School of Medicine, 40 Tsukasa-Machi, Gifu 500-8705, Japan
gifuim-gif{at}umin.u-tokyo.ac.jp
Objectives. We investigated expression of brain natriuretic peptide (BNP) as well as atrial natriuretic peptide (ANP) and their genes in human right atria. Their relations with atrial pressure were also examined.
Background. The BNP plays a roll in electrolyte-fluid homeostasis such as ANP. The tissue level is reported to be elevated in the failing ventricles. However, expression and transmural distribution of BNP in the atria remain unclear.
Methods. Expression of ANP and BNP was immunohistochemically investigated in the right atrial (RA) specimens from 21 patients who had undergone cardiac surgery. The mRNA of specimens were quantitatively measured by Northern blot analysis and also evaluated by in situ hybridization. In addition, plasma levels of ANP and BNP were measured in the patients.
Results. The BNP immunoreactivity was diffusely seen in RA tissue of patients with mean RA pressure (mRAP) of 5 mm Hg or more, but it was noted only in the subendocardial half of the atria of those with mRAP less than 5 mm Hg. There was a significant correlation between the incidence of BNP-positive myocytes and mRAP (r = 0.850, p < 0.0001). Conversely, ANP-positive myocytes were found diffusely in all cases. In Northern blot analysis, the mRNAs levels of ANP and BNP in the atrial tissue were positively correlated with the mRAP (ANP, p = 0.775, p < 0.005 and BNP, p = 0.771, p < 0.005). In situ hybridization confirmed these findings. The mRNA levels were significantly correlated to each other (r = 0.845, p < 0.0002). Plasma ANP and BNP levels were elevated in the patients compared with that in controls; however, none were significantly correlated with the mRAP.
Conclusions. Expression of BNP and BNP mRNA is augmented in the atria with increased pressure, and distributed predominantly in the subendocardial side. The level of BNP mRNA was well correlated with that of ANP mRNA. Thus, these two genes might be commonly regulated in response to atrial pressure.
|
Abbreviations and Acronyms
| | ANP | = atrial natriuretic peptide | | BNP | = brain natriuretic peptide | | CHF | = congestive heart failure | | mRAP | = mean right atrial pressure | | NYHA | = New York Heart Association | | RA | = right atria(l) |
|
This article has been cited by other articles:
|
|
|
|
 
B. Freestone, F. Gustafsson, A. Y. Chong, P. Corell, C. Kistorp, P. Hildebrandt, and G. Y. H. Lip
Influence of Atrial Fibrillation on Plasma Von Willebrand Factor, Soluble E-Selectin, and N-Terminal Pro B-type Natriuretic Peptide Levels in Systolic Heart Failure
Chest,
May 1, 2008;
133(5):
1203 - 1208.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Rienstra, I. C. Van Gelder, M. P. Van den Berg, F. Boomsma, and D. J. Van Veldhuisen
Natriuretic peptides in patients with atrial fibrillation and advanced chronic heart failure: determinants and prognostic value of (NT-)ANP and (NT-pro)BNP
Europace,
July 1, 2006;
8(7):
482 - 487.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Iwasaki, A. Kawamoto, M. Ishikawa, A. Oyamada, S. Nakamori, H. Nishimura, K. Sadamoto, M. Horii, T. Matsumoto, S. Murasawa, et al.
Dose-Dependent Contribution of CD34-Positive Cell Transplantation to Concurrent Vasculogenesis and Cardiomyogenesis for Functional Regenerative Recovery After Myocardial Infarction
Circulation,
March 14, 2006;
113(10):
1311 - 1325.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kiji, Y. Dohi, S. Takasawa, H. Okamoto, A. Nonomura, and S. Taniguchi
Activation of regenerating gene Reg in rat and human hearts in response to acute stress
Am J Physiol Heart Circ Physiol,
July 1, 2005;
289(1):
H277 - H284.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. E. Hopkins, Z. Chen, N. K. Fukagawa, C. Hall, H. J. Knot, and M. M. LeWinter
Increased Atrial and Brain Natriuretic Peptides in Adults With Cyanotic Congenital Heart Disease: Enhanced Understanding of the Relationship Between Hypoxia and Natriuretic Peptide Secretion
Circulation,
June 15, 2004;
109(23):
2872 - 2877.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. P. van den Berg, I. C. van Gelder, and D. J. van Veldhuisen
Depletion of atrial natriuretic peptide during longstanding atrial fibrillation
Europace,
January 1, 2004;
6(5):
433 - 437.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. W. Biondo, E. J. Ehrhart, D. D. Sisson, B. J. Bulmer, H. S. A. De Morais, and P. F. Solter
Immunohistochemistry of Atrial and Brain Natriuretic Peptides in Control Cats and Cats with Hypertrophic Cardiomyopathy
Vet. Pathol.,
September 1, 2003;
40(5):
501 - 506.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Ruskoaho
Cardiac Hormones as Diagnostic Tools in Heart Failure
Endocr. Rev.,
June 1, 2003;
24(3):
341 - 356.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Jourdain, M. Bellorini, F. Funck, Y. Fulla, N. Guillard, J. Loiret, B. Thebault, N. Sadeg, and M. Desnos
Short-term effects of sinus rhythm restoration in patients with lone atrial fibrillation: a hormonal study
Eur J Heart Fail,
June 1, 2002;
4(3):
263 - 267.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. M. Bialik, Z. A. Abassi, I. Hammel, J. Winaver, and D. Lewinson
Evaluation of Atrial Natriuretic Peptide and Brain Natriuretic Peptide in Atrial Granules of Rats with Experimental Congestive Heart Failure
J. Histochem. Cytochem.,
October 1, 2001;
49(10):
1293 - 1300.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. R Kalra, S. D Anker, and A. J.S Coats
Water and sodium regulation in chronic heart failure: the role of natriuretic peptides and vasopressin
Cardiovasc Res,
August 15, 2001;
51(3):
495 - 509.
[Full Text]
[PDF]
|
|
|
|
