Induction of cytokine expression in leukocytes in acute myocardial infarction
N Marx,
FJ Neumann,
I Ott,
M Gawaz,
W Koch,
T Pinkau,
and
A Schomig
Deutsches Herzzentrum und 1. Medizinische Klinik, Technische Universitat Munchen, Germany. marx@med1.med.tu-muenchen.de
OBJECTIVES: This study sought to investigate whether cytokine expression in leukocytes may be induced by plasma from the reperfused heart of patients with an acute myocardial infarction (MI). BACKGROUND: Reperfusion in acute MI is associated with deleterious local and systemic inflammatory responses that are regulated by cytokines. Induction of cytokine expression in resident leukocytes could contribute to inflammatory responses of the ischemic and reperfused heart. METHODS: Blood samples of 10 patients with an acute MI were obtained simultaneously from the coronary sinus and the aorta before and 5 min after recanalization of the coronary occlusion. Ten patients with elective percutaneous transluminal coronary angioplasty served as a control group. We incubated leukocytes from healthy donors with plasma samples and analyzed mRNA expression of interleukin (IL)-1 beta, IL-6, IL-8 and tumor necrosis factor-alpha (TNF-alpha) by Northern blot analysis. RESULTS: In patients with an acute MI, plasma obtained from the coronary sinus after recanalization increased the mRNA expression of IL-1 beta and IL-8 compared with that of plasma before recanalization (median [quartiles] difference before vs. after recanalization: 34.5 [4, 137], p = 0.017, for IL-1 beta; 18.5 [4, 35], p = 0.032, for IL-8) and simultaneously obtained aortic plasma (median [quartiles] coronary sinus-aortic differences after recanalization: 45.5 [-3, 115], p = 0.021, for IL-1 beta; 16 [4, 52], p = 0.005, for IL-8). No induction of IL-6 and TNF-alpha expression could be observed. No changes found in the study patients were detectable in the control group. CONCLUSIONS: Plasma from the ischemic and reperfused heart stimulates the expression of IL-1 beta and IL-8 in leukocytes. Therefore, leukocyte-derived cytokines may contribute to the regulation of cardiac inflammatory responses in patients with an acute MI.
This article has been cited by other articles:

|
 |

|
 |
 
S. Aker, C. Bantis, P. Reis, N. Kuhr, C. Schwandt, B. Grabensee, P. Heering, and K. Ivens
Influence of interleukin-6 G-174C gene polymorphism on coronary artery disease, cardiovascular complications and mortality in dialysis patients
Nephrol. Dial. Transplant.,
September 1, 2009;
24(9):
2847 - 2851.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. W. Calvert, S. Gundewar, M. Yamakuchi, P. C. Park, W. M. Baldwin III, D. J. Lefer, and C. J. Lowenstein
Inhibition of N-Ethylmaleimide Sensitive Factor Protects Against Myocardial Ischemia/Reperfusion Injury
Circ. Res.,
December 7, 2007;
101(12):
1247 - 1254.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Gundewar, J. W. Calvert, J. W. Elrod, and D. J. Lefer
Cytoprotective effects of N,N,N-trimethylsphingosine during ischemia- reperfusion injury are lost in the setting of obesity and diabetes
Am J Physiol Heart Circ Physiol,
October 1, 2007;
293(4):
H2462 - H2471.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Satoh, Y. Shimoda, T. Akatsu, Y. Ishikawa, Y. Minami, and M. Nakamura
Elevated circulating levels of heat shock protein 70 are related to systemic inflammatory reaction through monocyte Toll signal in patients with heart failure after acute myocardial infarction
Eur J Heart Fail,
December 1, 2006;
8(8):
810 - 815.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. Tan, Z. Ma, L. Han, R. Du, L. Zhao, X. Wei, D. Hou, B. H. Johnstone, M. R. Farlow, and Y. Du
Caffeic acid phenethyl ester possesses potent cardioprotective effects in a rabbit model of acute myocardial ischemia-reperfusion injury
Am J Physiol Heart Circ Physiol,
November 1, 2005;
289(5):
H2265 - H2271.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S.-i. Kawachi, N. Takeda, A. Sasaki, Y. Kokubo, K. Takami, H. Sarui, M. Hayashi, N. Yamakita, and K. Yasuda
Circulating Insulin-Like Growth Factor-1 and Insulin-Like Growth Factor Binding Protein-3 Are Associated With Early Carotid Atherosclerosis
Arterioscler Thromb Vasc Biol,
March 1, 2005;
25(3):
617 - 621.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Madjid, I. Awan, J. T. Willerson, and S. W. Casscells
Leukocyte count and coronary heart disease: Implications for risk assessment
J. Am. Coll. Cardiol.,
November 16, 2004;
44(10):
1945 - 1956.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. Francis, Y. Chu, A. K. Johnson, R. M. Weiss, and R. B. Felder
Acute myocardial infarction induces hypothalamic cytokine synthesis
Am J Physiol Heart Circ Physiol,
June 1, 2004;
286(6):
H2264 - H2271.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Bertuglia, A. Giusti, and P. Del Soldato
Antioxidant activity of nitro derivative of aspirin against ischemia-reperfusion in hamster cheek pouch microcirculation
Am J Physiol Gastrointest Liver Physiol,
March 1, 2004;
286(3):
G437 - G443.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
F Cipollone, M Fazia, A Iezzi, B Pini, F Costantini, D De Cesare, L Paloscia, G Materazzo, E D'Annunzio, T Bucciarelli, et al.
High preprocedural non-HDL cholesterol is associated with enhanced oxidative stress and monocyte activation after coronary angioplasty: possible implications in restenosis
Heart,
July 1, 2003;
89(7):
773 - 779.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
B. Schieffer
Interaction of interleukin-6 and angiotensin II in atherosclerosis: culprit for inflammation?
Eur. Heart J. Suppl.,
January 1, 2003;
5(suppl_A):
A25 - A30.
[Abstract]
[PDF]
|
 |
|

|
 |

|
 |
 
M. S. Sabatine, D. A. Morrow, C. P. Cannon, S. A. Murphy, L. A. Demopoulos, P. M. DiBattiste, C. H. McCabe, E. Braunwald, and C. M. Gibson
Relationship between baseline white blood cell count and degree of coronary artery disease and mortality in patients with acute coronary syndromes: A TACTICS-TIMI 18 substudy
J. Am. Coll. Cardiol.,
November 20, 2002;
40(10):
1761 - 1768.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Takahashi, J. Nishihira, M. Shimpo, Y. Mizue, S. Ueno, H. Mano, E. Kobayashi, U. Ikeda, and K. Shimada
Macrophage migration inhibitory factor as a redox-sensitive cytokine in cardiac myocytes
Cardiovasc Res,
December 1, 2001;
52(3):
438 - 445.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. Lango, L. Anisimowicz, J. Siebert, J. Rogowski, A. Bakowska, P. Mrozinski, and M. Narkiewicz
IL-8 concentration in coronary sinus blood during early coronary reperfusion after ischemic arrest
Eur. J. Cardiothorac. Surg.,
September 1, 2001;
20(3):
550 - 554.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. V. Barron, C. P. Cannon, S. A. Murphy, E. Braunwald, and C. M. Gibson
Association Between White Blood Cell Count, Epicardial Blood Flow, Myocardial Perfusion, and Clinical Outcomes in the Setting of Acute Myocardial Infarction : A Thrombolysis In Myocardial Infarction 10 Substudy
Circulation,
November 7, 2000;
102(19):
2329 - 2334.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y Hojo, U Ikeda, T Katsuki, O Mizuno, H Fukazawa, K Kurosaki, H Fujikawa, and K Shimada
Interleukin 6 expression in coronary circulation after coronary angioplasty as a risk factor for restenosis
Heart,
July 1, 2000;
84(1):
83 - 87.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
P. M. Ridker, N. Rifai, M. Pfeffer, F. Sacks, S. Lepage, and E. Braunwald
Elevation of Tumor Necrosis Factor-{alpha} and Increased Risk of Recurrent Coronary Events After Myocardial Infarction
Circulation,
May 9, 2000;
101(18):
2149 - 2153.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
B. Schieffer, E. Schieffer, D. Hilfiker-Kleiner, A. Hilfiker, P. T. Kovanen, M. Kaartinen, J. Nussberger, W. Harringer, and H. Drexler
Expression of Angiotensin II and Interleukin 6 in Human Coronary Atherosclerotic Plaques : Potential Implications for Inflammation and Plaque Instability
Circulation,
March 28, 2000;
101(12):
1372 - 1378.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
D. Zohlnhofer, K. Brand, K. Schipek, G. Pogatsa-Murray, A. Schomig, and F.-J. Neumann
Adhesion of Monocyte Very Late Antigen-4 to Endothelial Vascular Cell Adhesion Molecule-1 Induces Interleukin-1{beta}-Dependent Expression of Interleukin-6 in Endothelial Cells
Arterioscler Thromb Vasc Biol,
February 1, 2000;
20(2):
353 - 359.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Shibata, K. Ueshima, M. Harada, M. Nakamura, K. Hiramori, S. Endo, N. Sato, H. Mukaida, T. Suzuki, T. Suzuki, et al.
Effect of Magnesium Sulfate Pretreatment and Significance of Matrix Metalloproteinase-1 and Interleukin-6 Levels in Coronary Reperfusion Therapy for Patients with Acute Myocardial Infarction
Angiology,
July 1, 1999;
50(7):
573 - 582.
[Abstract]
[PDF]
|
 |
|

|
 |

|
 |
 
W.-L. Lee, J.-W. Chen, C.-T. Ting, S.-J. Lin, and P. H. Wang
Changes of the Insulin-Like Growth Factor I System during Acute Myocardial Infarction: Implications on Left Ventricular Remodeling
J. Clin. Endocrinol. Metab.,
May 1, 1999;
84(5):
1575 - 1581.
[Abstract]
[Full Text]
|
 |
|

|
 |

|
 |
 
M. W. Irwin, S. Mak, D. L. Mann, R. Qu, J. M. Penninger, A. Yan, F. Dawood, W.-H. Wen, Z. Shou, and P. Liu
Tissue Expression and Immunolocalization of Tumor Necrosis Factor-{alpha} in Postinfarction Dysfunctional Myocardium
Circulation,
March 23, 1999;
99(11):
1492 - 1498.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
D. K. Ledford
Immunologic Aspects of Vasculitis and Cardiovascular Disease
JAMA,
December 10, 1997;
278(22):
1962 - 1971.
[Abstract]
[PDF]
|
 |
|
|